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作者以人肺癌细胞Pc-10株和用腮腺炎病毒持续感染的Pc-10细胞(Pc-10/MpV株)作为检定细胞毒性作用的靶细胞。将此两种细胞用铬~(51)标记后与人外周血单核细胞于37℃培育16小时,测定从靶细胞释放的铬~(51)。研究结果表明,在细胞表面和细胞浆内含有腮腺炎病毒抗原的Pc-10/MpV细胞,比未感染病毒的pc-10细胞更易被带有腮腺炎病毒中和抗体供血者的外周血单核细胞所溶
The authors used Pc-10 human lung cancer cells and Pc-10 cells (Pc-10 / MpV strain) sustained infection with mumps virus as the target cells for cytotoxicity assay. The two kinds of cells were incubated with human peripheral blood mononuclear cells at 37 ° C for 16 hours after being labeled with chromium ~ (51), and the release of chromium ~ (51) from target cells was measured. The results show that Pc-10 / MpV cells containing mumps virus antigens on the cell surface and in the cytoplasm are more susceptible to peripheral blood mononuclear cells with mumps neutralizing antibody donors than pc-10 cells not infected with the virus Cell dissolved