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目的探讨脆性组氨酸三联体基因与喉癌的相关性,为临床诊断与治疗喉癌提供较为可靠的临床依据。方法研究组与对照组患者均进行喉黏膜活体检测,从而研究脆性组氨酸三联体基因在人体喉黏膜中第5外显子与第8外显子的缺失情况,并对检测结果进行记录,给予统计学分析,得出结论。结果研究组与对照组患者均进行喉黏膜活体检测可知,对照组23例正常人群检测结果脆性组氨酸三联体基因在人体喉黏膜中第5外显子与第8外显子无1例发生纯合性缺失现象,均在喉黏膜中成功扩增出脆性组氨酸三联体基因在人体喉黏膜中第5外显子与第8外显子,缺失率为0.00%;而研究组23例喉癌患者检测结果脆性组氨酸三联体基因在人体喉黏膜中第5外显子纯合性缺失人数为7例(30.43%),第8外显子纯合性缺失人数为8例(34.78%)。研究组与对照组患者脆性组氨酸三联体基因在人体喉黏膜中第5外显子与第8外显子纯合性缺失率对比结果具有明显差异,且P<0.05,差异具有统计学意义。结论第5外显子以及第8外显子的缺失可能对喉癌患者脆性组氨酸三联体基因抑制癌功能丧失具有密切关系,从而可进一步对临床研究激活脆性组氨酸三联体基因抑制癌功能的方法以及提高喉癌患者的治疗效果具有重要意义。
Objective To investigate the relationship between the gene of fragile histidine triad and laryngeal cancer and provide a reliable clinical basis for clinical diagnosis and treatment of laryngeal cancer. Methods Both the study group and the control group were tested for laryngeal mucosal biopsy in order to study the deletion of exon 5 and exon 8 in human laryngeal mucosa and to test the detection results. Give statistical analysis, draw conclusions. Results Both the study group and the control group performed laryngeal mucosal biopsies. The results of 23 normal subjects in the control group showed that none of the 5 exon and 8 exon in the human laryngeal mucosa Homozygous deletion phenomenon, were successfully amplified in the laryngeal mucosal fragile histidine triad gene in human laryngeal mucosa exon 5 and exon 8, the deletion rate was 0.00%; while the study group of 23 patients The number of homozygous deletions of exon 5 in human laryngeal mucosa was 7 cases (30.43%) in the detection of laryngeal cancer and the number of homozygous deletion in exon 8 was 8 cases (34.78%) %). There was significant difference in homozygous deletion rate of the fifth exon and exon 8 between the study group and the control group in the human laryngeal mucosa, P <0.05, the difference was statistically significant . Conclusions The deletion of exon 5 and exon 8 may be closely related to the suppression of the loss of cancer function in the patients with laryngeal cancer by using the fragile histidine triad gene. Therefore, it is possible to further investigate the clinical significance of activating fragile histidine triad gene Functional methods and improve the therapeutic effect of laryngeal cancer patients is of great significance.