自Barneet等(1961)明确地把研究瘤胃微生物营养与代谢的方法分为体内法和体外法以来,体外法已经成为研究瘤胃微生物代谢的主要方法。体外法也称瘤胃模拟培养法或人工瘤胃法,即把瘤胃内容物与底物在体外共同培养,研究微生物的活动和底物利用。它的特点是受实验动物的限制少,反应条件易控制,适于实验室的常 Since Barneet et al. (1961) unequivocally divided the methods of studying rumen microbial nutrition and metabolism into in vivo and in vitro methods, in vitro methods have become the primary method of studying rumen microbial metabolism. In vitro method is also called rumen simulated culture method or artificial rumen method, that is, rumen content and substrate co-culture in vitro, the study of microbial activity and substrate utilization. It is characterized by limited experimental animals less easy to control the reaction conditions, suitable for laboratory often