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目的 :研究氯沙坦和卡维地洛对缺血再灌注心肌细胞凋亡的影响 ,比较氯沙坦和卡维地洛对缺血再灌注心肌损伤的保护作用。方法 :结扎Wistar大鼠左冠状动脉前降支 ,建立大鼠缺血再灌注动物模型 ,采用原位末端标记细胞凋亡法检测心肌细胞凋亡 ,并利用光学显微镜进行细胞计数。结果 :单纯缺血 再灌注组心肌细胞凋亡数较假手术组明显增多 (37.5 3± 9.2 2 /视野∶0 .18± 0 .0 9/视野 ,P <0 .0 5 ) ,氯沙坦和卡维地洛组心肌细胞凋亡数分别为 8.74± 3.5 1/视野和 7.6 3± 4 .0 5 /视野 ,较单纯缺血 再灌注组明显减少 (P <0 .0 5 ) ,氯沙坦和卡维地洛两组间无明显区别 (P >0 .0 5 )。结论 :氯沙坦和卡维地洛对缺血再灌注心肌细胞损伤具有相似的保护作用 ,可明显减少缺血再灌注心肌细胞凋亡
AIM: To investigate the effects of losartan and carvedilol on cardiomyocyte apoptosis induced by ischemia-reperfusion in order to compare the protective effects of losartan and carvedilol on myocardial injury induced by ischemia-reperfusion. Methods: The left anterior descending coronary artery was ligated in Wistar rats to establish the rat model of ischemia-reperfusion. The apoptosis of cardiomyocytes was detected by in situ terminal labeling of apoptotic cells, and the cells were counted by light microscope. Results: Compared with sham operation group, the number of apoptotic cardiomyocytes in ischemia-reperfusion group was significantly increased (37.5 3 ± 9.2 2 / field of view: 0.18 ± 0.09 / field, P <0.05), losartan And carvedilol group were 8.74 ± 3.51 / field and 7.63 ± 4.05 / field, respectively, which were significantly lower than those in ischemia-reperfusion group (P <0.05) Tan and carvedilol no significant difference between the two groups (P> 0.05). Conclusion: Losartan and carvedilol have a similar protective effect on myocardial cell injury induced by ischemia-reperfusion, and can significantly reduce the apoptosis of myocardial cells during ischemia-reperfusion