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以往的实验表明,横纹肌可通过直接注射DNA表达质粒后转基因并有效表达产物。但横纹肌内不含有如树突状细胞,巨噬细胞及淋巴细胞等免疫细胞而不能提呈抗原并激活免疫系统。由于皮肤及粘膜的特殊解剖位置是许多抗原免括一些病毒首先接触的部位,表皮及真皮下突含的淋巴细胞中含有大量的免疫细胞,这些细胞将抗原捕捉后,处理并提呈给T细胞,激活免疫系统。皮下注射DNA后,转染靶细胞,其表达的基因产物可激活免疫系统,这为抗病毒感染免疫提供一条有效的基因免疫途径。
Previous experiments have shown that striated muscle can be genetically modified by direct injection of DNA into a plasmid and expressed efficiently. However, striated muscle does not contain such as dendritic cells, macrophages and lymphocytes and other immune cells can not be presented antigen and activate the immune system. Due to the special anatomical location of the skin and mucous membrane is a number of antigens from some of the virus first contact with the site, epidermal and subepithelial lymphocytes contain a large number of immune cells that capture the antigen and then presented to T cells , Activate the immune system. Subcutaneous injection of DNA, transfected target cells, the expression of the gene product can activate the immune system, which provides an effective immune response to viral infection of the genetic route.