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目的 探讨依达拉奉对大鼠肝缺血再灌注(I/R)损伤中的保护作用.方法 将60只大鼠随机分为实验组和对照组,建立常温下部分肝脏I/R动物模型.在肝脏I/R开始时和1 h后对实验组大鼠给子依达拉奉注射液,对照组给予同等容量的生理盐水.再灌注0,2,4 h测定肝脏脂质过氧化反应物(LPO)浓度和肝脏酶学指标及TNF-α和E-selectin的mRNA,并行两组肝脏的病理学检查.结果 再灌注2,4 h实验组大鼠肝脏LPO反应程度和肝脏酶指标检测值明显低于对照组(P＜0.05).再灌注2 h肝TNF-αmRNA和E-selectin mRNA表达明显低于对照组(P＜0.05).再灌注2 h实验组大鼠肝脏切片的E-seleetin免疫反应性明显低于对照组.结论 依达拉奉能抑制氧化应激反应,从而降低肝I/R损伤;并显著减少炎性细胞和黏附分子的产生,抑制炎性反应的发生.“,”Objective To investigate the protective effect of edaravone on ischemia reperfusion injury (IRI) in rat liver.Methods Sixty rats were randomly divided into experimental group and controll groups (30 in each) after establishing animal liver IRI model with partial reperfusion injury under normal temperature.Just after initiation of reperfusion and 1 h later,edaravone was administered in the experiment group,and the same volume of normal saline was administered in the control group.The lipid peroxidation (LPO) hepatic enzymes and the level of TNF-α mRNA and E-selectin mRNA in plasma were measured at 0,2,4 h after initiation of reperfusion.We also serially quantified hepatic expression of mRNAs for TNF-α and E-selectin with RT-PCR.Results In the experiment group,hepatic LPO and hepatic enzyme were significantly less than that in the saline group(P ＜ 0.05) at 2 h after initiation of reperfusion.Hepatic expression of TNF-α and E-selectin mRNA was significantly lower in the experiment group than the saline group(P＜0.05) at 2h after initiation of reperfusion.Histologically,E-selectin expression was less evident in hepatic sections in the experiment group than controll group at 2h after initiation of reperfusion.Conclusions Edaravone can reduce hepatic ischemia reperfusion injury,and significangly inhibit subsequent inflammation by reducing expression of inflammatory cytokines and adhesion molecules.