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高卢蜜环菌(Armillaria gallica)为北半球广布种,不同大陆间的菌株遗传相似性和多样性水平能反映出该种在洲际大陆尺度上的地理遗传变异关系。作者用ISSR(inter-simple sequence repeat)分子标记技术,对从中国和欧洲收集到的高卢蜜环菌79个菌株进行了遗传多样性分析。用6个ISSR引物扩增得到210个位点,其中多态性位点(频率<0.95)为202个,占96.2%,平均每个引物多态位点多达33.6个,表明ISSR标记在蜜环菌中存在较高的多态性。根据非加权类平均法(UPGMA)聚类分析,中国53个菌株中的49个在0.773的相似性水平上聚成了中国类群(China group);而欧洲26个菌株遗传分化较大,分别在0.775和0.763的相似性水平上聚成了欧洲类群A(Europe group A)和B(Europe group B);2个欧洲类群间的相似性水平仅为0.738,而欧洲类群A与中国类群间的相似性却达到了0.770;两个大陆均有少数菌株表现出较为明显的遗传分化,个别菌株的种内遗传相似性甚至低于蜜环菌种间的遗传相似性。结果表明,中欧两个大陆间的A.gallica菌株因地理隔离已经表现出明显的遗传分化,处于异域物种形成过程中;欧洲大陆的菌株遗传分化更为明显,可能是两个大陆A.gallica菌株的起源地。
Armillaria gallica is a widely distributed species in the northern hemisphere. The genetic similarity and diversity of isolates from different continents reflect the genetic variation of this species at the intercontinental scale. The authors used the ISSR (inter-simple sequence repeat) molecular marker technique to analyze the genetic diversity of 79 strains of G. arvense collected from China and Europe. There were 210 polymorphic loci amplified by 6 ISSR primers, of which 202 polymorphic loci (frequency <0.95) accounted for 96.2%, with an average of 33.6 polymorphic loci per primer, indicating that the ISSR markers were in honey There is a high degree of polymorphism in the bacteria. According to the UPGMA cluster analysis, 49 of the 53 Chinese strains were clustered into the Chinese group at a similarity level of 0.773. However, the genetic differentiation of 26 isolates in Europe was significant 0.775 and 0.763. The similarity between the two European groups was only 0.738, while the similarity between the European group A and the Chinese group was similar Sexuality reached 0.770; a few strains in both continents showed more obvious genetic differentiation, and the intraspecific genetic similarity of individual strains was even lower than the genetic similarity among Armillaria species. The results showed that the A.gallica strain in the two continents of Central Europe showed obvious genetic differentiation due to geographical isolation and was in the process of the formation of exotic species. The genetic differentiation of the strains in continental Europe was more obvious, which may be caused by A.gallica strain Origin.