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目的 :探讨不同组织学分型的脊索瘤的临床病理特征。方法 :分析临床资料 ,运用HE ,糖原染色(PAS) ,alcianblue染色 ,免疫组织化学染色和电镜技术观察 17例脊索瘤的病理切片。其中经典型脊索瘤 11例 ,软骨样脊索瘤 6例。结果 :所有脊索瘤都发生在中轴骨骼 ,均含有空泡细胞。瘤细胞胞浆和细胞外基质PAS ,alcianblue着色。免疫组织化学染色 ,肿瘤联合表达上皮性标记和S 10 0蛋白。超微结构见瘤细胞胞浆空泡、线粒体—粗面内质网复合体和细胞间桥粒样连接。结论 :根据组织学、免疫组织化学染色和超微结构特征 ,结合临床和放射影像学资料 ,可以确诊脊索瘤。
Objective: To investigate the clinicopathological features of chordomas with different histological types. METHODS: Clinical data were analyzed and HE lesions, glycogen staining (PAS), alcianblue staining, immunohistochemical staining and electron microscopy were used to observe the pathological sections of 17 cases of chordoma. Of these, 11 cases were classical chordomas and 6 cases were cartilage-like chordomas. Results: All chordomas occurred in the axial skeleton and contained vacuolar cells. Tumor cells cytoplasm and extracellular matrix PAS, alcianblue staining. Immunohistochemically, the tumors were combined to express epithelial markers and S 10 0 proteins. Ultrastructure showed cytoplasmic vacuoles, mitochondria-matrix endoplasmic reticulum complexes, and intercellular bridge-like connections. Conclusion: According to histological, immunohistochemical staining and ultrastructural features combined with clinical and radiological data, chordoma can be diagnosed.