构建 pGEX4T 1 HPV16L1重组表达系统 ,为进一步研制基因工程疫苗奠定基础 ,采用PCR方法扩增HPV16中国分离株L1外源基因片段 ,pGEX4T 1为表达载体 ,构建pEGX4T 1 HPV16L1重组表达系统 ,在大肠杆菌宿主BL(2 1)中 ,经IPTG诱导 ,表达融合蛋白GST L1,经SDS PAGE电泳和Westernblot进行鉴定结果表明 ,成功构建pEGX4T 1 HPV16L1重组表达质粒并获得高效表达并经Westernblot鉴定为L1外源蛋白。提示 :所构建的 pGEX4T 1 HPV16L1重组表达质粒能够高效表达HPV16L1蛋白 The pGEX4T 1 HPV16L1 recombinant expression system was constructed to lay the foundation for the further development of genetic engineering vaccines. PCR was used to amplify the exogenous gene fragment of HPV16 Chinese isolate L1. pGEX4T1 was used as the expression vector to construct the recombinant expression system of pEGX4T 1 HPV16L1 in Escherichia coli host. In BL (21), the fusion protein GST L1 was induced by IPTG and identified by SDS PAGE electrophoresis and Western blot. The results showed that the recombinant plasmid pEGX4T 1 HPV16L1 was successfully constructed and expressed highly and identified as L1 exogenous protein by Western blot. Tip : The constructed pGEX4T 1 HPV16L1 recombinant expression plasmid can efficiently express HPV16L1 protein