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[目的]建立并评估大鼠钳夹式脊髓损伤模型,为研究急性脊髓损伤的机制及后续治疗提供基础。[方法]40只成年S-D大鼠,随机分为脊髓损伤组和假手术组,脊髓损伤组行T10水平的椎板切除术,并用一动脉瘤夹瞬间释放,造成脊髓的急性挫伤,假手术组大鼠则仅行T10水平的椎板切除,术后进行行为学评价,血清学检测及病理学检查。[结果]脊髓损伤组大鼠术后运动功能评分(BBB评分)低于假手术组大鼠,术后4 h血清TNF-α和IL-6的含量高于假手术组,且两组差异具有统计学意义,病理学检查脊髓损伤组有脊髓实质结构破坏、空洞及瘢痕形成,假手术组未见明显异常。[结论]本研究建立的大鼠急性脊髓损伤模型,能够反映脊髓损伤的病理生理过程,并具有较好的重复性及稳定性,可用于脊髓损伤的研究。
[Objective] To establish and evaluate a rat model of spinal cord injury and provide a basis for studying the mechanism and follow-up of acute spinal cord injury. [Methods] Forty adult Sprague-Dawley rats were randomly divided into spinal cord injury group and sham operation group. The spinal cord injury group underwent T10 level laminectomy and an instantaneous release of an aneurysm clip resulted in spinal cord acute contusion. Only T10 level of laminectomy rats, postoperative behavioral evaluation, serological testing and pathological examination. [Results] The scores of motor function score (BBB score) in rats with spinal cord injury group were lower than those in sham operation group, and the levels of serum TNF-α and IL-6 in 4 h after operation were higher than those in sham operation group Statistical significance, pathological examination spinal cord injury group spinal cord parenchyma structure destruction, cavities and scar formation, no significant abnormalities in the sham operation group. [Conclusion] The rat model of acute spinal cord injury established in this study can reflect the pathophysiological process of spinal cord injury and has good repeatability and stability and can be used for the study of spinal cord injury.