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目的:检测星形细胞肿瘤组织中FOXP3蛋白的表达,探讨星形细胞肿瘤组织中FOXP3的表达与临床病理特征以及预后的关系。方法:应用免疫组织化学技术检测121例星形细胞肿瘤和5例脑膜瘤,5例正常脑组织中FOXP3的表达。分析FOXP3的表达与星形细胞肿瘤各临床病理参数及预后的关系。结果:FOXP3蛋白在人脑星形细胞肿瘤组织中有表达,在脑膜瘤和正常脑组织中均未见表达,FOXP3蛋白主要表达于间质淋巴细胞的细胞核。在星形细胞肿瘤中,FOXP3的表达在各级别星形细胞肿瘤中表达差异显著(P<0.05)。Kaplan-Meier生存分析提示FOXP3阳性组和阴性组生存时间差异显著(P<0.01),但COX多因素变量分析结果显示,FOXP3不是影响星形细胞肿瘤患者术后生存时间的独立预后因素(P>0.05)。结论:FOXP3在星形细胞肿瘤间质淋巴细胞中的表达与星形细胞肿瘤的恶性程度以及病人的年龄相关。FOXP3阳性的星形细胞肿瘤的预后较FOXP3阴性的星形细胞肿瘤差,但不能作为影响星形细胞肿瘤生存时间的独立预后因素。FOXP3可能成为星形细胞肿瘤免疫调节的新靶点。
Objective: To detect the expression of FOXP3 protein in astrocytic tumors and to investigate the relationship between FOXP3 expression and clinicopathological features and prognosis in astrocytoma. Methods: Immunohistochemistry was used to detect the expression of FOXP3 in 121 cases of astrocytoma, 5 cases of meningioma and 5 cases of normal brain tissue. The relationship between FOXP3 expression and clinicopathological parameters and prognosis of astrocytoma was analyzed. Results: FOXP3 protein was expressed in human brain astrocytoma tissue, but not in meningiomas and normal brain tissues. FOXP3 protein was mainly expressed in the nucleus of interstitial lymphocytes. In astrocytic tumors, the expression of FOXP3 was significantly different in all grades of astrocytic tumors (P <0.05). Kaplan-Meier survival analysis showed significant differences in survival time between FOXP3 positive group and negative group (P <0.01). However, COX multivariate analysis showed that FOXP3 was not an independent prognostic factor of survival time in astrocytoma patients (P> 0.05). CONCLUSIONS: The expression of FOXP3 in stromal lymphocytes of astrocytic tumors correlates with the malignancy of astrocytic tumors and the patient’s age. The prognosis of FOXP3-positive astrocytic tumors is worse than that of FOXP3-negative astrocytic tumors, but not as an independent prognostic factor for the survival of astrocytic tumors. FOXP3 may be a new target of immune regulation of astrocytic tumors.