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目的观察腹主动脉内灌注瑞芬太尼聚己内酯(REM-PCL)对兔脊髓缺血再灌注中神经细胞线粒体损伤的影响。方法 20只健康新西兰大白兔随机分为对照组(C组)及REM-PCL组(RP组,0.1mg/kg),每组10只。采用肾下腹主动脉阻断法,建立脊髓缺血再灌注损伤(SCIRI)模型。分别于缺血前、缺血45min、再灌注30min和60min时,测定脊髓神经细胞线粒体超氧化物歧化酶(SOD)、活性氧(ROS)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、总抗氧化能力(T-AOC)、线粒体肿胀度(MSD),并观察其病理学改变。结果与缺血前比较,C组阻断腹主动脉后脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均降低(P<0.01),ROS、MDA和MSD含量均升高;开放腹主动脉后C组脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均降低(P<0.01),ROS、MSD和MDA含量均升高(P<0.01),脊髓灰质病理损害严重(P<0.01);RP组在阻断腹主动脉45min时和开放腹主动脉后脊髓神经细胞线粒体SOD、GSH-PX及T-AOC均显著高于C组(P<0.01),ROS、MSD和MDA含量均显著低于C组(P<0.01),RP组脊髓灰质的病理损害程度明显轻于C(P<0.01)。结论 SCIRI中腹主动脉内灌注REM-PCL可提高神经细胞线粒体抗氧化能力,减轻神经细胞线粒体损伤。
Objective To observe the effect of remifentanil poly-L-caprolactone (PPAR-β) infused into the abdominal aorta on the mitochondrial damage induced by spinal cord ischemia-reperfusion in rabbits. Methods Twenty healthy New Zealand white rabbits were randomly divided into control group (C group) and REM-PCL group (RP group, 0.1 mg / kg), with 10 rabbits in each group. The model of spinal cord ischemia-reperfusion injury (SCIRI) was established by subrenal abdominal aorta occlusion. The activities of mitochondrial superoxide dismutase (SOD), reactive oxygen species (ROS) and glutathione peroxidase (GSH-PX) in spinal cord neurons were measured before ischemia, 45 min after ischemia, 30 min and 60 min after reperfusion, , Malondialdehyde (MDA), total antioxidant capacity (T-AOC), mitochondrial swelling (MSD), and observed the pathological changes. Results Compared with pre-ischemic group, the mitochondrial SOD, GSH-PX and T-AOC of spinal cord neurons in C group were decreased (P <0.01), ROS, MDA and MSD were increased in C group; The levels of SOD, GSH-PX and T-AOC in the spinal cord neurons were decreased (P <0.01), the content of ROS, MSD and MDA in spinal cord neurons were increased in group C (P <0.01) In the RP group, mitochondrial SOD, GSH-PX and T-AOC in the spinal cord neurons were significantly higher than those in the C group (P <0.01) at 45 min after the abdominal aorta was occluded and the abdominal aorta was opened. The contents of ROS, MSD and MDA in the RP group were significantly lower In group C (P <0.01), the degree of pathological damage of gray matter in RP group was significantly lower than that in C group (P <0.01). Conclusion Infusion of REM-PCL in the middle abdominal aorta of SCIRI can improve mitochondrial antioxidant capacity and mitochondrial damage of nerve cells.