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目的探讨 HDV/ HBV感染树鼠句肝组织中 Fas/ Fas L、Bcl2 / Bax和 ICE表达与 HDV感染之间的关系 ,以及Fas/ Fas L、Bcl2 / Bax和 ICE在丁型肝炎肝细胞凋亡中的作用。方法采用免疫组化技术对 HDV/ HBV感染树鼠句肝组织中 Fas/Fas L、Bcl2 / Bax和 ICE的表达进行检测 ;应用原位末端标记技术对肝细胞凋亡进行检测。结果 HDAg表达与 Fas/ Fas L、Bcl2 /Bax和 ICE表达之间关系密切 ( P<0 .0 5 ) ,HDAg表达越强 ,Fas、Fas L、Bax和 ICE表达也越强 ,而 Bcl2 表达则越弱。 Fas/Fas L、Bcl2 / Bax和 ICE表达与肝细胞凋亡之间关系密切 ( P<0 .0 5 ) ,Fas、Fas L、Bax和 ICE表达越强 ,凋亡细胞越多 ;相反 ,Bcl2 表达越强 ,凋亡细胞越少。结论肝细胞内 HDAg表达可诱导 Fas、Fas L、Bax和 ICE表达 ,但对 Bcl2 表达无明显诱导作用 ;Fas/ Fas L、Bcl2 / Bax和 ICE在肝细胞凋亡中起重要作用。
Objective To investigate the relationship between the expression of Fas / Fas L, Bcl2 / Bax and ICE in HDV / HBV infected rat liver tissue and HDV infection, and the effects of Fas / Fas L, Bcl2 / Bax and ICE on hepatocyte apoptosis in hepatitis D In the role. Methods The expression of Fas / Fas L, Bcl2 / Bax and ICE in liver tissue of HDV / HBV infected rats were detected by immunohistochemistry. Apoptosis of hepatocytes was detected by in situ end labeling. Results The expression of HDAg was closely related to the expression of Fas / Fas L, Bcl2 / Bax and ICE (P <0.05). The stronger the expression of HDAg, the stronger the expression of Fas, Fas L, Bax and ICE, while the expression of Bcl2 The weaker. The expressions of Fas / Fas L, Bcl2 / Bax and ICE were closely related to the apoptosis of hepatocytes (P <0.05), the stronger the expression of Fas, Fas L, Bax and ICE, the more apoptotic cells; The stronger the expression, the less apoptotic cells. Conclusion HDGF expression in hepatocytes induces expression of Fas, Fas L, Bax and ICE, but has no obvious effect on Bcl2 expression. Fas / Fas L, Bcl2 / Bax and ICE play an important role in hepatocyte apoptosis.