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目的了解抗日战争期间丽水地区细菌战幸存者鼠疫耶尔森菌和炭疽杆菌感染情况。方法鼠疫感染幸存者血清通过间接血凝法检测鼠疫F1抗体。炭疽感染幸存者创口涂抹物通过荧光定量PCR法和胶体金法检测炭疽杆菌抗原。结果 106份鼠疫感染幸存者血清鼠疫F1抗体阳性9份,滴度均≥1∶32,滴度最高为1∶512,阳性率为8.49%,均通过间接血凝抑制试验验证。16份炭疽患者创口涂抹物标本2种检测方法结果均为阴性。结论鼠疫感染幸存者血清中F1抗体阳性,说明感染鼠疫后患者体内产生的抗体可长时间或终身存在。炭疽感染幸存者患皮肤炭疽,百治不愈,创口暂时无传染性,不会对当地居民构成较大的传染性危害。
Objective To understand the infection of Yersinia pestis and Bacillus anthracis against the germ-column survivors in Lishui during the Anti-Japanese War. Methods The serum of plague-infected survivors was tested for F1 antibody against plague by indirect hemagglutination. Anthrax infection survivors wound smears detected by fluorescence quantitative PCR and colloidal gold anthrax antigens. Results The positive rate of F1 antibody in 106 plague infected survivors was 9, the titer was 1: 32, the highest titer was 1: 512 and the positive rate was 8.49%, all of which were confirmed by indirect hemagglutination inhibition test. 16 samples of wound smear specimens of patients with anthrax two kinds of test results were negative. Conclusion The F1 antibody was positive in the serums of plague-infected survivors, indicating that the antibodies produced in patients after plague infection may exist for a long time or for a long time. Anthrax infected survivors of skin anthrax, Baizhuyu, wounds temporarily non-contagious, will not constitute a greater contagious harm to local residents.