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以红车轴草种子为试材,利用不同浓度的NaCl溶液、NaHCO_3溶液、NaCl和NaHCO_3(1∶1)混合溶液在培养皿中模拟3种盐碱环境,观察红车轴草种子的萌发状况,以期明确红车轴草的耐盐碱能力。结果表明:红车轴草种子发芽率、发芽势、相对发芽率、发芽指数、活力指数、苗鲜重和胚根长等指标,表现出随NaCl、NaHCO_3、NaCl和NaHCO_3混合溶液(1∶1)浓度的增加,总体呈下降趋势。苗鲜重和胚根长,呈现显著的负相关关系,相对盐害率与溶液浓度呈现正相关关系。NaCl胁迫下红车轴草种子萌发的耐盐浓度8.091‰,半数抑制浓度10.146‰,极限浓度12.200‰;NaHCO_3胁迫下红车轴草萌发的耐盐浓度5.483‰,半数抑制浓度7.434‰,极限浓度9.386‰;NaCl与NaHCO_3(1∶1)混合溶液胁迫下红车轴草萌发的耐盐浓度6.081‰,半数抑制浓度7.253‰,极限浓度8.425‰。蒸馏水复萌试验表明,经3种盐碱胁迫后红车轴草种子均有不同程度的复萌。综上所述,红车轴草种子具有一定的抗耐盐碱能力。
The seeds of red clover seeds were used as test materials to simulate three kinds of saline-alkali environment in petri dishes with different concentrations of NaCl solution, NaHCO 3 solution, NaCl and NaHCO 3 (1: 1) Clear red clover resistance to salt and alkali. The results showed that the germination rate, germination potential, relative germination rate, germination index, vigor index, seedlings fresh weight and radicle length of the seeds of T.mongolica showed that with the mixture of NaCl, NaHCO 3, NaCl and NaHCO 3 (1: 1) The increase of concentration showed an overall downward trend. Fresh weight and radicle length showed a significant negative correlation, the relative salt damage rate and the concentration of the solution showed a positive correlation. Under NaCl stress, the salt tolerance of T. mongolicum seed germination was 8.091 ‰, the median inhibitory concentration was 10.146 ‰, and the limit concentration was 12.200 ‰. The salt tolerance concentration of C. mongolicum under salt stress was 5.483 ‰, the half inhibitory concentration was 7.434 ‰ and the limit concentration was 9.386 ‰ Salt tolerance concentration of 6.081 ‰, half-concentration inhibitory concentration 7.253 ‰ and limit concentration 8.425 ‰ were observed under the stress of mixed solution of NaCl and NaHCO 3 (1: 1). Repeated watering distilled water test showed that after three kinds of salt-alkali stress Red clover seeds have varying degrees of Meng Meng. In summary, red clover seeds have some resistance to salt and alkali.