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目的观察伯氏疟原虫(Pb)ANKA株哌喹抗性系(PQR)感染小鼠组织病理学变化特征,比较与哌喹敏感系(PQS)的差异,探讨其抗性机制。方法实验小鼠随机分为A(PQS)、B(PQR)及C(正常对照,NC)三组,取肝、脾、脑及肾组织,10%福尔马林固定后石蜡包埋,4μm切片,苏木精一伊红(Haematoxylin-eosin,HE)及吉姆萨(Giemsa)染色,镜下观察其组织病理学改变。结果与PQS组比较,PQR组疟原虫感染红细胞(p RBC)及疟色素(HZ)在各器官组织中出现晚,增殖缓慢,数量明显偏少,在17d增至峰值时仍明显少于PQS组6d时,22d时开始下降。各组织的炎性病变随感染天数的延长而变化:早期较轻,呈渐进性发展,17d时达到高峰,22d时炎症减轻,组织损伤呈恢复趋势,其两组肝、脾组织中炎性细胞的浸润程度在6d时未见明显不同。脑组织的炎性反应、疟原虫浸润及组织损伤程度均显著轻于PQS组。结论 PQR系的毒力下降、致病力降低表象的组织病理学特征为:肝、脾、脑和肾组织中p RBC及HZ浸润量明显偏低,炎性反应、原虫数量的变化呈逐渐增加、降低、趋向恢复的动态过程,未见明显的脑型疟的组织学变化。
Objective To observe the histopathological changes of Plasmodium resistant (PQR) infected mice infected by Plasmodium berghei (Pb) ANKA and compare the difference with PQS and explore the mechanism of its resistance. Methods The experimental mice were randomly divided into three groups: A (PQS), B (PQR) and C (normal control, NC). The mice were fixed in 10% formalin and embedded in paraffin The sections were stained with hematoxylin-eosin (HE) and Giemsa to observe the histopathological changes. Results Compared with the PQS group, the P RBC and HZ of the PQR group appeared late in various organs and tissues. The proliferation rate of the PQR group was slower than that of the PQS group. The numbers of P RBC and HZ in the PQR group were significantly lower than those in the PQS group 6d, 22d began to decline. Inflammatory lesions in each tissue changed with the days of infection: the early was mild, the progressive development, reached its peak on the 17th day, the inflammation alleviated on the 22nd, and the tissue injury tended to recover. The inflammatory cells in the liver and spleen The degree of infiltration at 6d no significant difference. Inflammatory reaction, malaria parasite invasion and tissue damage in brain tissue were significantly lower than those in PQS group. Conclusions The pathological features of PQR-induced decreased virulence and reduced pathogenicity are as follows: the infiltration of p RBC and HZ in liver, spleen, brain and kidney tissues is significantly lower, and the inflammatory response and the number of protozoa gradually increase , Reduce, tend to restore the dynamic process, no obvious histological changes in cerebral malaria.