作者根据蜡状芽胞杆菌在某些组织培养系统中产生细胞毒作用的原理,将34株蜡状芽胞杆菌和25株其他芽胞杆菌的培养物上清液(CS)经过过滤灭菌,取0.2ml(1.5×10~4/ml)分别加于含有McCoy单层细胞培养物的96孔Linbro组织培养板的小孔中,一式三份,于35℃、5%CO_2下培养24h,在加入CS前与加入时以及1、3、5和24h,用倒置显微镜观察结果,记录每孔中细胞培养物的特征和形态学变化。 Based on the principle of Bacillus cereus producing cytotoxicity in some tissue culture systems, 34 strains of Bacillus cereus and 25 strains of Bacillus other culture supernatant (CS) were filtered and sterilized, and 0.2 ml (1.5 × 10-4 / ml) were added to wells of a 96-well Linbro tissue culture plate containing McCoy monolayer culture in triplicate and incubated at 35 ° C, 5% CO 2 for 24 h before addition of CS At the time of addition and at 1, 3, 5 and 24 h, the results were observed with an inverted microscope and the characteristics and morphological changes of the cell cultures in each well were recorded.