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从 gus基因的瞬时表达入手 ,利用不同种类的农杆菌菌株感染小麦不同基因型和同一基因型的不同外植体 ,研究了农杆菌菌系、小麦基因型和外植体对转化效率的影响。从中优选出了对小麦愈伤组织感染力比较强的农杆菌菌系 AGL 0和 MOG10 1,以及高敏感受体基因型 Alondra和扬麦 15 8等。实验结果还表明 ,预培养 10～ 15天的幼胚愈伤组织具有较高的瞬时表达率和再生能力 ,是较好的转化受体。对愈伤组织与农杆菌共培养后的筛选程序进行了摸索 ,获得了 70多个 PPT(Phosphinothricin)抗性植株 ,经 PCR等分子检测分析 ,证明部分植株实现了外源基因的成功转化 ,为进一步的研究工作打下了基础。
Starting from the transient expression of gus gene, different types of Agrobacterium strains were used to infect different explants of different genotypes and genotypes of wheat to study the effects of Agrobacterium strains, wheat genotypes and explants on transformation efficiency. The Agrobacterium strains AGL 0 and MOG10 1, which are relatively infestative to wheat callus, were optimized and the Alondra and Yangmai 15 8 genotypes were optimized. The experimental results also showed that pre-cultured immature embryos callus 10 to 15 days have a higher instantaneous expression rate and regeneration ability, is a better conversion receptor. More than 70 PPT (Phosphinothricin) -resistant plants were screened after the co-culture of the callus and Agrobacterium. PCR and other molecular analysis showed that some plants were successfully transformed with foreign genes Further research laid the foundation.