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目的血浆自身抗体是调控心力衰竭发生发展的重要因子。C10orf97基因与病理性心脏重构和心衰密切相关,但心衰患者血浆中是否存在抗C10orf97自身抗体目前完全未知。方法采用病例-对照研究策略,纳入107例心衰患者和163例正常对照。人工合成C10orf97三段亲水性多肽,用酶联免疫吸附法检测血浆C10orf97自身抗体水平。结果在正常对照的血浆中,C10orf97蛋白的三个自身抗体阳性率很低,仅为1.2%。但在心衰患者中,三个自身抗体的阳性率均显著升高,分别为27.1%、32.7%和29.9%,与对照组相比差异极其显著(所有的p<0.0001)。心衰组中三个自身抗体均为阳性的比率为17.8%,远高于对照组的0.6%(P<0.0001)。扩张型心肌病和非扩张型心肌病两个亚组的自身抗体阳性率无显著性差异,提示血浆C10orf97自身抗体水平升高与多种原因引起的心衰均相关。结论我们的研究首次揭示血浆C10orf97自身抗体与心衰密切相关,血浆C10orf97自身抗体很可能为新的介导心衰发展的自身免疫异常因子。
Objective Plasma autoantibodies are important factors in the development of heart failure. C10orf97 gene and pathological cardiac remodeling and heart failure are closely related, but the existence of plasma anti-C10orf97 autoantibodies in patients with heart failure is currently unknown. Methods A case-control study strategy was enrolled in 107 patients with heart failure and 163 normal controls. Synthetic C10orf97 three hydrophilic peptides, C10orf97 autoantibody levels by enzyme-linked immunosorbent assay. Results In the normal control plasma, the positive rate of the three autoantibodies of the C10orf97 protein was very low, only 1.2%. However, in patients with heart failure, the positive rates of the three autoantibodies were significantly elevated at 27.1%, 32.7% and 29.9%, respectively, with significant differences compared to the control group (all p <0.0001). Heart failure group in the three autoantibodies were positive for the ratio of 17.8%, much higher than the control group of 0.6% (P <0.0001). Dichotomizing cardiomyopathy and non-dilated cardiomyopathy two subgroups of autoantibodies the positive rate was no significant difference, suggesting that elevated plasma levels of C10orf97 autoantibodies and a variety of causes of heart failure are related. Conclusions Our study revealed, for the first time, that plasma C10orf97 autoantibodies are closely related to heart failure. Plasma C10orf97 autoantibodies are likely to be new autoimmune abnormalities that mediate the development of heart failure.