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目的研究虫草多糖对荷瘤小鼠T淋巴细胞功能的影响。方法除正常对照组外,其余小鼠右前肢腋窝皮下注射S180肉瘤细胞悬液建立小鼠荷瘤模型。荷瘤小鼠随机分为荷瘤对照(等容生理盐水)组、香菇多糖(1 mg·kg?1)组、虫草多糖高、中、低剂量(200,100,50 mg·kg?1)组,腹腔注射,每天1次,连续13 d后,检测虫草多糖对荷瘤小鼠的抑瘤作用以及荷瘤小鼠外周血T淋巴细胞及其亚群数量、Th1/Th2类细胞因子水平和脾淋巴细胞转化功能。结果虫草多糖高、中剂量组可明显抑制S180肉瘤生长,提高荷瘤小鼠脾脏重量和脾脏系数;提高外周血CD3+细胞(总T淋巴细胞)、CD4+CD8?亚群数量及CD4+CD8?/CD4?CD8+比值,提高Th1淋巴细胞分泌的细胞因子TNF-?和IL-2水平,并显著促进脾脏T淋巴细胞增殖转化能力。结论虫草多糖具有明显的抗肿瘤活性,其机制可能与调节机体T淋巴细胞及其亚群的数量与分泌功能有关。
Objective To investigate the effect of Cordyceps polysaccharide on the function of T lymphocytes in tumor-bearing mice. Methods In addition to the normal control group, the other mice were injected subcutaneously into the armpit of S180 sarcoma cell suspension in the right forelimb to establish a mouse tumor-bearing model. Tumor-bearing mice were randomly divided into the tumor-bearing control group (isocratogenic saline group), the group of lentinan (1 mg · kg -1), the group of high, medium and low doses of Cordyceps polysaccharide (200,100,50 mg · kg -1) The mice were injected intraperitoneally once daily for 13 days. The antitumor effects of Cordyceps polysaccharide on tumor-bearing mice and the numbers of T lymphocytes and their subsets, Th1 / Th2 cytokines and spleen lymphocytes Cell transformation function. Results Cordyceps polysaccharide high and medium dose groups can significantly inhibit the growth of S180 sarcoma, increase the spleen weight and spleen coefficient of tumor-bearing mice; increase the number of CD3 + cells (total T lymphocytes), CD4 + CD8 + subsets and CD4 + CD8 + / CD4 (superscript +) CD8 (superscript +) ratio increased the levels of TNF-|Áand IL-2 secreted by Th1 lymphocytes and promoted the proliferation and transformation of T lymphocytes. Conclusion Cordyceps polysaccharides have obvious anti-tumor activity, and its mechanism may be related to regulating the number and secretion of T lymphocytes and their subpopulations.