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目的探讨血小板膜糖蛋白(GP)Ⅰa基因和Ⅰbα基因多态性与脑梗死发生的关系,为缺血性脑卒中的预防及治疗提供理论基础。方法选择经CT或MRI证实的脑梗死患者302例(脑梗死组)和健康体检者196例(对照组);采用PCR-RFLP方法检测GPⅠa C807T基因与Ⅰbα基因HPA-2、Kozak序列多态性在2组中的分布频率。结果脑梗死组GPⅠa C807T等位基因频率明显高于对照组,差异有统计学意义(P<0.05);脑梗死组GPⅠbα基因Kozak序列C等位基因频率明显高于对照组差异有统计学意义(25.33% vs 10.20%,P<0.05);脑梗死组GPⅠbα基因HPA-2序列等位基因频率、基因型与对照组比较,差异无统计学意义(P>0.05)。结论 GⅠbα基因HPA-2序列多态性与脑梗死无相关性;GPⅠa C807T等位基因和Ⅰbα基因Kozak序列多态性可能是脑梗死的遗传危险因素。
Objective To investigate the relationship between platelet glycoprotein (GP) Ⅰ a gene and Ⅰ bα gene polymorphism and the occurrence of cerebral infarction, and to provide a theoretical basis for the prevention and treatment of ischemic stroke. Methods 302 patients with cerebral infarction (cerebral infarction group) and 196 healthy controls (control group) confirmed by CT or MRI were selected. The GPⅠa C807T gene and Ⅰbα gene HPA-2, Kozak sequence polymorphism Distribution frequency in 2 groups. Results The frequency of GPⅠa C807T allele in cerebral infarction group was significantly higher than that in control group (P <0.05). The frequency of C allele of GP Ⅰ bα gene Kozak sequence in cerebral infarction group was significantly higher than that in control group 25.33% vs 10.20%, P <0.05). There was no significant difference in the frequencies and genotypes of GP Ⅰ bα gene HPA-2 in cerebral infarction group compared with the control group (P> 0.05). Conclusion There is no correlation between HPA-2 polymorphism of GⅠbα gene and cerebral infarction. Polymorphism of GPⅠa C807T allele and Ⅰbα gene Kozak sequence may be the genetic risk factors of cerebral infarction.