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在pH 8.5~9.5的Britton-Robinson缓冲溶液中,健那绿与硫酸软骨素作用形成结合产物时导致溶液共振瑞利散射(RRS)显著增强并产生新的RRS光谱,其最大散射峰位于326 nm处,另在407 nm和560 nm处有两个强度较小的散射峰。硫酸软骨素在0.1~5.0μg/ml浓度范围内与RRS强度的线性关系良好。其最低检测限为25.3 ng/ml。
In Britton-Robinson buffer solution of pH 8.5 ~ 9.5, the conjugation product of jian-green with chondroitin sulfate resulted in a significant increase of Resonance Rayleigh Scattering (RRS) and new RRS spectra, with the maximum scattering peak located at 326 nm At the same time, there are two smaller intensity peaks at 407 nm and 560 nm. Chondroitin sulfate has a good linear relationship with the RRS intensity in the concentration range of 0.1 ~ 5.0μg / ml. The minimum detection limit is 25.3 ng / ml.