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目的 :观察白细胞介素 12真核表达载体 (pWRG316 9)对HBV -SDNA疫苗 (pCR3.1-S)诱导Balb/c小鼠 (H - 2d)的特异性细胞免疫应答及其对稳定表达HBsAg的小鼠肥大细胞瘤P815细胞 (P815 -HBV -S)成瘤性的影响。方法 :肌肉注射DNA疫苗及白细胞介素 12真核表达载体 ,背部皮下接种P815 -HBV -S细胞 ,观察成瘤情况 ,4h51Cr释放法检测小鼠脾细胞CTL活性。结果 :对照组成瘤率 10 0 % ,pCR3·1-S组小鼠成瘤率为 12 .5 % ,pCR3·1-S +IL - 12真核表达载体组成瘤率为 0 ,对照组平均存活期和 6周后存活率分别为 2 8.4d和 0。后两组分别大于 38.2d及 45d ,存活率为 87.5 %及 10 0 % ,pCR3·1-S组CTL细胞杀伤活性 5 1.1% ,联合白细胞介素 12真核表达载体组 72 .6 % ,对照组为 2 0 .5 % (P <0 .0 1)。结论 :DNA疫苗可以诱导小鼠细胞免疫应答 ,对体内HBV感染具有预防及治疗作用。白细胞介素 12真核表达载体可加强DNA疫苗的上述作用
Objective: To observe the specific cellular immune response of interleukin - 12 (pWRG316 9) to Balb / c mice (H - 2d) induced by HBV DNA vaccine (pCR3.1-S) and its effect on the stable expression of HBsAg On the tumorigenicity of mouse mastocytoma P815 cells (P815-HBV-S). Methods: The DNA vaccine and interleukin 12 eukaryotic expression vector were intramuscularly injected. P815-HBV-S cells were inoculated subcutaneously in the back and the tumorigenicity was observed. The CTL activity of splenocytes was detected by 4h51Cr release assay. Results: The control group had a tumorigenic rate of 10%. The rate of tumorigenesis in the pCR3 · 1-S group was 12.5%, and that of the pCR3 · 1-S + IL-12 eukaryotic expression vector was 0, Survival rates at 2 weeks and 6 weeks were 2 8.4d and 0 respectively. The survival rates of the two groups were greater than 38.2d and 45d, respectively. The survival rates were 87.5% and 100% respectively. The killing activity of CTL cells in pCR3 · 1-S group was 51.1% and that of IL-12 eukaryotic expression vector group was 72.6% The group was 20.5% (P <0. 01). Conclusion: DNA vaccine can induce cellular immune response in mice and has the preventive and therapeutic effects on HBV infection in vivo. The interleukin 12 eukaryotic expression vector enhances the above effects of the DNA vaccine