论文部分内容阅读
目的探讨异丙酚预处理对急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)大鼠多器官Gq/11蛋白表达的影响及其保护作用。方法 24只雄性Wistar大鼠随机分为油酸组、预处理组和对照组。油酸组由大鼠尾静脉注射油酸0.2 ml/kg(2 min内)复制ARDS模型。预处理组经左颈静脉输注异丙酚80 mg.kg-1.h-1,30 min后再以相同方式注射油酸。对照组从尾静脉注射同剂量的生理盐水。测定各组大鼠平均动脉压(MABP)、血气、血浆和各器官乳酸脱氢酶(lactate dehydrogenase,LDH)、丙二醛(malondialdehyde,MDA)、血管紧张素转换酶(angiotensin converting enzyme,ACE)含量。免疫印迹法检测各器官Gq/11蛋白含量。结果油酸组和预处理组的pH、PaO2较对照组下降(P<0.05)。与对照组相比,油酸组血浆与肺、脑、心、肾、肝、小肠以及预处理组心、肾、小肠LDH活性明显升高(P<0.05)。预处理组血浆、肺、脑、心、肾、肝LDH活性较油酸组降低(P<0.05)。与对照组相比,油酸组血浆、肺、脑、心、肾、肝、小肠以及预处理组血浆、心、肾MDA含量明显升高(P<0.05)。预处理组血浆、肺、脑、心、肾、肝、小肠MDA含量较油酸组降低(P<0.05)。与对照组相比,油酸组和预处理组血浆、肺脏ACE活性明显降低(P<0.05),而肾脏、小肠ACE活性升高(P<0.05)。与油酸组比较,预处理组血浆ACE活性升高(P<0.05),肺脏、小肠ACE活性降低(P<0.05)。油酸组肺、脑、心、肾、肝、小肠以及预处理组肺、脑、心、肾、小肠中Gαq/11蛋白表达较对照组明显升高(P<0.05)。与油酸组比较,预处理组肺、脑、肾、肝中的Gαq/11蛋白表达有所降低(P<0.05),心、小肠的变化无明显差异(P>0.05)。结论异丙酚预处理能减轻ARDS时多器官损伤程度,其抑制Gq/11蛋白高表达变化可能是其保护途径之一。
Objective To investigate the effect of propofol preconditioning on Gq / 11 protein expression in multiple organs of acute respiratory distress syndrome (ARDS) rats and its protective effect. Methods Twenty-four male Wistar rats were randomly divided into oleic acid group, pretreatment group and control group. In the oleic acid group, ARDS model was replicated by injecting oleic acid 0.2 ml / kg (within 2 min) into the tail vein of rats. Propofol was injected into the left jugular vein 80 mg.kg-1.h-1,30 min after pretreatment, and oleic acid was injected in the same way. Control group from the tail vein injection of the same dose of saline. Mean arterial pressure (MABP), blood gas, plasma and various organs lactate dehydrogenase (LDH), malondialdehyde (MDA), angiotensin converting enzyme (ACE) content. Western blotting was used to detect Gq / 11 protein content in each organ. Results The pH and PaO2 of oleic acid group and pretreatment group were lower than those of the control group (P <0.05). Compared with the control group, the activities of LDH in heart, kidney, small intestine and the plasma of the oleic acid group were significantly increased (P <0.05) compared with those in the lung, brain, heart, kidney, liver, small intestine and preconditioning group. LDH activity in plasma, lung, brain, heart, kidney, liver in pretreatment group was lower than that in oleic acid group (P <0.05). Compared with the control group, the contents of MDA in plasma, heart, kidney, liver, small intestine and pretreatment group were significantly increased in oleic acid group (P <0.05). The content of MDA in plasma, lung, brain, heart, kidney, liver and small intestine in pretreatment group was lower than that in oleic acid group (P <0.05). Compared with the control group, ACE activity in plasma and lung of oleic acid group and pretreatment group decreased significantly (P <0.05), but ACE activity in kidney and small intestine increased (P <0.05). Compared with the oleic acid group, the plasma ACE activity increased (P <0.05) in the pretreatment group and decreased in the lung and small intestine (P <0.05). The expression of Gαq / 11 protein in lung, brain, heart, kidney, liver, small intestine and preconditioning group of oleic acid group was significantly higher than that of control group (P <0.05). Compared with the oleic acid group, the Gαq / 11 protein expression in the lung, brain, kidney and liver of the pretreatment group was decreased (P <0.05), but there was no significant difference in the heart and small intestine (P> 0.05). Conclusion Propofol pretreatment can reduce the degree of multiple organ damage in ARDS, which may be one of its protective ways by inhibiting the high expression of Gq / 11 protein.