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目的:观察miR-194模拟物对于成骨肉瘤细胞系SOSP_9607细胞增殖、周期和凋亡的影响。方法:四甲基偶氮唑蓝(MTT)法绘制细胞生长曲线,流式细胞仪测定细胞凋亡和周期。将SOSP_9607细胞分为对照组和实验组,对照组分为阴性对照和正常细胞对照组。实验组采用miR-194模拟物(hsa-miR-194mimics)转染成骨肉瘤细胞系SOSP_9607,增强SOSP_9607细胞内miR-194的活性。结果:与对照组比较,实验组细胞的增殖能力明显下降。实验组凋亡率(10.1±0.22)%与阴性对照组凋亡率(3.3±0.19)%相比明显增高(P<0.01)。与对照组比较,实验组细胞周期G0/G1细胞比例显著增加,G2/M期细胞比例显著减少,S期细胞比例显著减少(P<0.01)。结论:通过转染miR-194模拟物增强SOSP_9607细胞中miR-194的活性对SOSP_9607细胞的增殖和凋亡造成显著影响。
OBJECTIVE: To observe the effect of miR-194 mimics on proliferation, cycle and apoptosis of osteosarcoma cell line SOSP_9607. Methods: MTT method was used to draw the cell growth curve. Flow cytometry was used to detect apoptosis and cell cycle. SOSP_9607 cells were divided into control group and experimental group, the control group was divided into negative control group and normal cell control group. The experimental group was transfected into osteosarcoma cell line SOSP_9607 using miR-194 mimics (hsa-miR-194mimics) to enhance the activity of miR-194 in SOSP_9607 cells. Results: Compared with the control group, the proliferation ability of the experimental group cells decreased significantly. The apoptosis rate in the experimental group (10.1 ± 0.22)% was significantly higher than that in the negative control group (3.3 ± 0.19)% (P <0.01). Compared with the control group, the proportion of G0 / G1 cells in the experimental group was significantly increased, the proportion of cells in G2 / M phase was significantly decreased, and the proportion of cells in S phase was significantly decreased (P <0.01). CONCLUSIONS: The enhanced miR-194 activity in SOSP_9607 cells transfected with miR-194 mimics significantly affects the proliferation and apoptosis of SOSP_9607 cells.