目的:研究Skp2、c-myc及p27蛋白在口腔鳞癌组织中的表达。方法:免疫组化检测Skp2、c-myc及p27蛋白在54例口腔鳞癌和15例正常口腔黏膜组织中的表达。结果:口腔鳞癌组织中Skp2、c-myc阳性表达率分别为35.19%(19/54)和38.89%(21/54),显著高于正常口腔黏膜组织6.67%(1/15)、6.67%(1/15)(P<0.05);阳性表p27达率为55.56%(30/54)显著低于正常口腔黏膜组织86.67%(13/15)(P<0.05);Skp2的表达与口腔鳞癌的临床分期、病理分级、淋巴结转移显著相关(P<0.05),与年龄、性别、肿瘤部位等因素无关(P>0.05);口腔鳞癌中Skp2与c-myc表达呈正相关(r=0.562,P<0.01);与p27蛋白的表达呈负相关(r=-0.532,P<0.01)。结论:Skp2蛋白过表达在口腔鳞癌的发生及发展中起重要作用;并可能与c-myc蛋白有协同作用,Skp2蛋白过表达与靶蛋白p27蛋白降解有关,联合检测Skp2、c-myc及p27蛋白的表达有助于综合评估口腔鳞癌的生物学行为。 Objective: To study the expression of Skp2, c-myc and p27 in oral squamous cell carcinoma. Methods: The expressions of Skp2, c-myc and p27 in 54 cases of oral squamous cell carcinoma and 15 cases of normal oral mucosa were detected by immunohistochemistry. Results: The positive rates of Skp2 and c-myc in oral squamous cell carcinoma were 35.19% (19/54) and 38.89% (21/54) respectively, which were significantly higher than those in normal oral mucosa (6.67%, 1/15) and 6.67% (1/15) (P <0.05). The positive rate of p27 was 55.56% (30/54) in the positive group, which was significantly lower than that in the normal oral mucosa (86.67%, 13/15) The clinical stage, pathological grade and lymph node metastasis were significantly correlated (P <0.05), but not with age, sex, tumor location and other factors (P> 0.05). The positive expression of Skp2 and c-myc in oral squamous cell carcinoma , P <0.01), but negatively correlated with the expression of p27 protein (r = -0.532, P <0.01). Conclusion: Skp2 overexpression plays an important role in the carcinogenesis and development of oral squamous cell carcinoma. It may play synergistic effect with c-myc protein. The overexpression of Skp2 protein is related to the degradation of p27 protein. The combination of Skp2, c-myc and The expression of p27 protein helps to evaluate the biological behavior of oral squamous cell carcinoma comprehensively.