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目的:探讨游泳训练对大鼠非酒精性脂肪性肝炎形成的影响。方法:30只雄性SD大鼠随机分为4组:对照组(正常饮食12周)、高脂饮食组(高脂饮食12周)、高脂饮食运动组(高脂饮食同时游泳训练12周)和运动组(正常饮食同时游泳训练12周)。12周后处死大鼠,观察各组肝脏组织切片并测试血清以及肝脏各项指标。结果:高脂饮食组大鼠肝脏出现严重的大泡性脂肪变性并有不同程度的炎性细胞浸润,高脂饮食运动组肝脏偶有脂肪变性和炎性细胞,对照组和运动组为正常肝脏组织形态。高脂饮食组的肝脏脂肪变性程度和炎症活动度评分均显著高于对照组,而高脂饮食运动组显著低于高脂组、显著高于对照组。高脂饮食组血清和肝脏游离脂肪酸、血清甘油三酯、肿瘤坏死因子α、肝脏丙二醛含量均显著高于对照组,而高脂饮食运动组显著低于高脂组;高脂饮食运动组血清和肝脏游离脂肪酸、肿瘤坏死因子α、肝脏丙二醛含量与对照组相比无显著性差异;高脂饮食组血清胆固醇显著高于对照组,而肝脏超氧化物歧化酶显著低于对照组,两者与高脂饮食运动组无显著性差异;高脂饮食运动组血清甘油三酯和胆固醇显著高于对照组,而超氧化物歧化酶显著低于对照组。结论:运动训练可以在一定程度上预防非酒精性脂肪性肝炎的形成。
Objective: To investigate the effect of swimming training on the formation of nonalcoholic steatohepatitis in rats. Methods: Thirty male SD rats were randomly divided into 4 groups: control group (normal diet for 12 weeks), high fat diet group (high fat diet for 12 weeks), high fat diet exercise group (high fat diet for 12 weeks swimming training) And exercise group (normal diet while swimming training for 12 weeks). After 12 weeks, the rats were sacrificed and the liver tissue sections of each group were observed and the serum and liver indexes were tested. Results: The rats in the high-fat diet group had severe bullous steatosis and inflammatory cell infiltration in the liver, while the occasional steatosis and inflammatory cells in the high-fat diet group were normal. The control group and exercise group were normal liver Organizational form. The degree of fatty degeneration and the score of inflammatory activity in the high-fat diet group were significantly higher than those in the control group, while the high-fat diet exercise group was significantly lower than the high-fat diet group, significantly higher than the control group. The content of free fatty acid, serum triglyceride, tumor necrosis factor α and liver MDA in the high fat diet group were significantly higher than those in the control group, while the high fat diet exercise group was significantly lower than the high fat diet group. The high fat diet exercise group Serum and liver free fatty acids, tumor necrosis factor α, liver MDA content of the control group showed no significant difference; high-fat diet serum cholesterol was significantly higher than the control group, while the liver superoxide dismutase was significantly lower than the control group , Respectively. There was no significant difference between the two groups and the high-fat diet exercise group. Serum triglycerides and cholesterol in the high-fat diet exercise group were significantly higher than those in the control group, while superoxide dismutase was significantly lower than the control group. Conclusion: Exercise training can prevent the formation of non-alcoholic steatohepatitis to a certain extent.