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目的研究和厚朴酚、青蒿素及二者联合应用对人肺腺癌SPC-A-1细胞生长的抑制作用。方法 MTT法测定和厚朴酚、青蒿素单独或联合对细胞生长的抑制作用,以IC50评判抗肿瘤的效果,应用金氏公式进行联合用药分析的结果;流式细胞仪检测和厚朴酚、青蒿素单独或联合作用对SPC-A-1细胞周期分布的影响。结果和厚朴酚能显著抑制SPC-A-1细胞的增殖,并呈剂量依赖关系,其半数抑制浓度(IC50)为8.20μg.mL-1;青蒿素对SPC-A-1细胞生长没有明显的抑制作用,半数抑制浓度(IC50)为53.52μg.mL-1;和厚朴酚与青蒿素联合应用对SPC-A-1细胞的增殖抑制作用优于各单药组,Q>1.15。细胞周期分布显示联合用药组将细胞阻滞于G1期,显著高于各单药组。结论和厚朴酚单独能够杀伤SPC-A-1细胞,并呈量-效关系,青蒿素单独作用不杀伤SPC-A-1细胞,两者青蒿素联合应用对SPC-A-1细胞具有协同作用。
Objective To study the inhibitory effects of honokiol, artemisinin and their combination on the growth of human lung adenocarcinoma SPC-A-1 cells. METHODS MTT assay was used to determine the inhibitory effect of honokiol and artemisinin alone or in combination on the cell growth. IC50 was used to evaluate the anti-tumor effect. The results were analyzed by the combination of King’s formula and flow cytometry. , Artemisinin alone or in combination on the cell cycle distribution of SPC-A-1. Results Honokiol could significantly inhibit the proliferation of SPC-A-1 cells in a dose-dependent manner with the IC50 of 8.20μg · mL-1. Artemisinin had no effect on the growth of SPC-A-1 cells IC50 was 53.52μg.mL-1. The inhibitory effect of honokiol and artemisinin on the proliferation of SPC-A-1 cells was better than that of single drug group (Q> 1.15) . The cell cycle distribution showed that the combination group blocked the cells in G1 phase, which was significantly higher than that in the single drug group. Conclusions Honokiol can kill SPC-A-1 cells alone, and in a dose-effect relationship, the combination of artemisinin and SPC-A-1 cells Has synergy.