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目的:探讨荔枝核皂苷对D-半乳糖诱致小鼠学习记忆障碍的改善作用。方法:50只昆明种小鼠完全随机分为正常对照组、模型对照组、石杉碱甲组(0.4 mg/kg·d)、荔枝核皂苷低(15 g/kg·d)和高(60 g/kg·d)剂量组。上述动物除空白对照组外,均皮下(sc)注射D-半乳糖(500 mg/kg·d)8周,并于第5周起分别灌胃相应药物。采用水迷宫和自主活动仪测试小鼠学习和记忆能力,测定血清糖基化;醛糖还原酶活性;采用高效液相库仑阵列电化学检测系统检测人神经氨酸酶(NA)、多巴胺(DA)、5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)二羟苯乙酸(DOPAC)的含量变化。结果:荔枝核皂苷明显改善模型小鼠学习和记忆能力,各给药组与模型对照组相比单胺类递质含量差异无统计学意义(P>0.05)。结论:荔枝核皂苷具有改善D-半乳糖诱致小鼠学习记忆障碍的作用;其作用可能与其减少抑制对脑神经细胞的损伤作用有关。
Objective: To investigate the effect of lychee nucleoside on D-galactose induced learning and memory impairment in mice. Methods: Fifty Kunming mice were randomly divided into normal control group, model control group, huperzine A group (0.4 mg / kg · d), low (15 g / kg · d) and high g / kg · d) dose group. The above animals were injected with D-galactose (500 mg / kg · d) for 8 weeks except for the blank control group, and the corresponding drugs were respectively orally administered in the fifth week. The learning and memory abilities of mice were measured by water maze test and autonomic activity meter. Serum glycosylation and aldose reductase activity were measured. High-performance liquid Coulometric array electrochemical detection system was used to detect the contents of human neuraminidase (NA), dopamine ), Serotonin (5-HT) and 5-hydroxyindoleacetic acid (5-HIAA) and diphenyloxanilic acid (DOPAC) Results: Litchi nuclear saponin significantly improved the ability of learning and memory of model mice. There was no significant difference in the content of monoamine neurotransmitters between the two groups (P> 0.05). Conclusion: Litchi saponin can improve the learning and memory impairment induced by D-galactose in mice, which may be related to its effect on reducing the damage of brain nerve cells.