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目的:探讨PKC在脑损伤后血脑屏障损害中的作用。方法:雄性SD大鼠60只,随机分为正常对照组、假手术组、脑损伤组和H7治疗组。治疗组于伤后0.5h腹腔注射PKC阻断剂H7 1mg/kg,12h重复注射一次,荧光显微镜定性观察,分光光度计定量检测伊文氏兰渗出。结果:脑损伤后伊文氏兰明显渗出,治疗组渗出明显减少。结论:应用PKC阻断剂H7能明显阻止血脑屏障开放,表明PKC在脑损伤后血脑屏障损害中起重要作用。
Objective: To investigate the role of PKC in the damage of blood-brain barrier after brain injury. Methods: Sixty male SD rats were randomly divided into normal control group, sham operation group, brain injury group and H7 treatment group. The rats in the treatment group were intraperitoneally injected with 1 mg / kg PKC blocker H7 at 0.5h after injury, and were injected repeatedly 12h after injection. Qualitative observation was carried out by fluorescence microscope, and the effusion of Evans blue was quantitatively detected by spectrophotometer. Results: Evans blue significantly exuded after brain injury, and the exudation of the treatment group was significantly reduced. Conclusion: The application of PKC blocker H7 can obviously prevent the opening of the blood-brain barrier, indicating that PKC plays an important role in the damage of the blood-brain barrier after brain injury.