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目的:研究红景天苷在大鼠小肠上吸收饱和现象和钠依赖性葡萄糖糖转运体(SGLT1)是否参与其的吸收。方法:测定大鼠灌胃12,24和48mg·kg-1红景天苷后的血药浓度-时间数据和估算相应药物代谢动力学参数。分析在SGLT1的抑制剂根皮苷存在情况下红景天苷在大鼠空肠的肠外囊中摄取,研究在链脲佐菌素诱导的糖尿病大鼠吸收动力学,并用在体肠灌流技术测定红景天苷在空肠中的有效渗透系数。结果:大鼠灌胃24和48mg·kg-1红景天苷后,它们的血药浓度-时间曲线几乎重合,两种剂量的药物代谢动力学参数也相似,提示吸收存在饱和现象。肠外翻实验结果显示红景天苷的吸收是浓度依赖性的,根皮苷浓度依赖性抑制红景天苷的吸收。红景天苷在糖尿病大鼠胃肠道的吸收快于正常大鼠,且其有效渗透系数也高。结论:红景天苷在大鼠小肠上存在吸收饱和现象,SGLT1可能参与红景天苷的吸收调节。
Objective: To study the salinity uptake of salidroside in rat intestine and the role of sodium-dependent glucose transporter (SGLT1) in its absorption. Methods: The plasma concentration-time data of 12, 24 and 48 mg · kg-1 salidroside groups were measured and the corresponding pharmacokinetic parameters were estimated. Analysis of salidroside uptake in the intestinal jejunum of rat jejuni in the presence of the inhibitor phlorizin SGLT1 to study the kinetics of absorption in streptozotocin-induced diabetic rats and its use in the determination of the intestinal perfusion technique Effective penetration coefficient of salidroside in jejunum. RESULTS: After administration of salidroside at 24 and 48 mg · kg-1, the plasma concentration-time curves of rats were almost coincident with each other. The pharmacokinetic parameters of the two doses were also similar, indicating that there was saturation in the absorption. Parenteral test results show that salidroside absorption is concentration-dependent, and phlorizin inhibits salidroside absorption in a concentration-dependent manner. Salidroside in the gastrointestinal tract of diabetic rats absorbed faster than normal rats, and its effective permeability coefficient is also high. Conclusion: Salidroside can absorb saturate in rat intestine. SGLT1 may be involved in the regulation of salidroside absorption.