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本文应用流式细胞术(FCM)和Feulgen-DNA法显示分裂指数,分析山豆根对Eca-109细胞的作用。流式细胞分析结果表明,实验组细胞DNA指数(DI=2)显著低于对照组(DI=2.5),两者相比差异均有极其显著性意义(P<0.01)。细胞DNA组方图G_0/G_1期细胞呈增高趋势,S期细胞均减少,G_2M期细胞均增高,与对照组比差异均有显著性(P<0.01,P<0.05)。Feulgen-DNA染色显示细胞分裂指数与小剂量药物作用时间呈负相关,大剂量药物作用下分裂指数明显下降。这说明山豆根对Eca-109细胞DNA合成有显著的抑制作用。
In this paper, flow cytometry (FCM) and Feulgen-DNA methods were used to show the splitting index and the effect of Shandou root on Eca-109 cells was analyzed. Flow cytometry analysis showed that the DNA index (DI=2) was significantly lower in the experimental group than in the control group (DI=2.5), and the difference between the two groups was extremely significant (P<0.01). The cells in the G0/G1 phase of the DNA group showed a tendency to increase, the cells in the S phase decreased, and the cells in the G2M phase increased. There was a significant difference compared with the control group (P<0.01, P<0.05). Feulgen-DNA staining showed that the cell division index was negatively correlated with the time of low-dose drug action, and the splitting index was significantly decreased under high-dose drug treatment. This shows that Shandougen has a significant inhibitory effect on DNA synthesis of Eca-109 cells.