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利用海藻酸钠作为包埋剂对苯系物细胞传感器Pseudomonas fluorescens A506(pTS)进行固定化处理,并对细胞浓度、固定化时间、投加量等检测因素进行了优化.固定化后的细胞与游离细胞对苯系污染物的检测效果进行对比.经过2 h固定化处理的细胞传感器,在检测时间1.5～6.0 h内,信号上升速度为游离细胞的2.26倍,信号最大值为游离细胞的2.23倍.固定化处理后的细胞生长缓慢,且浓度远低于游离细胞,说明了固定化处理后的单位细胞信号强度增加.同时,细胞浓度随着固定化时间的延长而降低.在影响因素方面,细胞浓度和固定化时间对于检测信号的影响较大.固定化后,对高浓度的苯系污染物有着更快的信号响应.
Pseudomonas fluorescens A506 (pTS) was immobilized by sodium alginate as an embedding agent, and the detection factors such as cell concentration, immobilization time and dosage were optimized.The immobilized cells were incubated with Free cells detected the effect of benzene-based pollutants.After 2 h immobilized cell sensor, the detection time of 1.5 ~ 6.0 h, the signal rise rate of 2.26 times the free cells, the maximum signal free cells 2.23 Fold.The immobilized cells grow slowly and at a much lower concentration than the free cells, indicating that the signal intensity of the unit cells after the immobilization treatment increases.At the same time, the cell concentration decreases with the extension of the immobilization time. , Cell concentration and immobilization time have a greater influence on the detection signal.After immobilization, it has a faster signal response to high concentrations of benzene-based pollutants.