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采集未感染HIV-1的孪生同胞的淋巴细胞,在添有5％胎牛血清(FCS)、抗CD3抗体和白细胞素-2(100IU/ml)的培养基中培养10天,然后输给HIV-1感染患者以试图恢复其免疫能力。间隔期为6～8周,在多次输入后,半数的患者在输入后的4～12小时内产生了类Arthus反应,持续39℃高热、低血压、寒战、关节痛、肌肉痛、头痛及全身不适。输入前和输入后患者的血清样本是否存在FCS抗体。结果所有输入前的血清均为阴性,而12例患者中有8例,经过两次或两次以上输入含FCS的培养基中培养的淋巴细胞后,产生了FCS抗体。标准的牛肉浸液划痕皮试所有患者均为阴性,CD4初始水平与FCS抗体的产生相关(在FCS抗体阳性患者中,初始CD4中间值=362.0/μl,而无反应患者的初始CD4中间
Lymphocytes from uninfected twin siblings of HIV-1 were harvested for 10 days in media supplemented with 5% fetal bovine serum (FCS), anti-CD3 antibody, and leukotrielin-2 (100 IU / ml) -1 infect patients in an attempt to regain their immunity. Intervals of 6 to 8 weeks resulted in an Arthus-like reaction within the first 4 to 12 hours after input, after repeated entry, lasting 39 ° C with fever, hypotension, chills, joint pain, muscle pain, headache and Unwell. Pre-infusion and post-infusion serum samples from patients with FCS antibodies. Results All pre-input sera were negative, whereas 8 of 12 patients developed FCS antibodies after two or more of the lymphocytes cultured in FCS-containing media. All patients were negative for the standard beef immersion scratch test and the initial level of CD4 was correlated with the production of FCS antibodies (median initial CD4 = 362.0 / μl in FCS antibody-positive patients, but not in patients with initial CD4 center