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从浙江某规模猪场分别采集不同日龄商品猪口腔液与血清样本,应用实时定量PCR和ELISA方法,分别检测2种体液中猪繁殖与呼吸综合征病毒(PRRSV)和猪圆环病毒2型(PCV2)的抗原和抗体水平,并分析其消长规律。结果提示:2种病毒各自的特异性抗体在口腔液和血清中的消长规律基本一致。相比血清,口腔液中的母源抗体存续时间更长(3周),主动免疫后产生特异性抗体的时间则更晚(3周)。口腔液中PRRSV和PCV2的感染动态规律,与血清和口腔液抗体的消长规律也具有很好的相关性,在出现病毒感染高峰后,血清和口腔液特异性抗体转阳,其中血清抗体的转阳反应更为迅速(3周)。本研究为进一步发展猪群口腔液检测系统提供了临床依据。
Porcine oral fluid and serum samples were collected from pigs of different sizes on a certain scale pig farm in Zhejiang Province. Real-time PCR and ELISA were used to detect the expression of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) antigen and antibody levels, and analyze its growth and decline. The results suggest that the specific antibodies of the two viruses are basically the same in the oral liquid and serum. Maternal antibodies in oral fluids last longer (3 weeks) than serum, and produce more specific antibodies after active immunization (3 weeks). The dynamic changes of PRRSV and PCV2 infection in oral fluid also had a good correlation with the growth and decay of serum and oral fluid antibody. After the peak of virus infection, the serum and oral fluid-specific antibodies turned positive. The serum antibody turnover Positive reaction is more rapid (3 weeks). This study provides a clinical basis for further development of swine oral fluid detection system.