论文部分内容阅读
以“红阳”猕猴桃茎段为外植体,MS为基本培养基,培养温度25±2℃,光照强度4 500lx,16h/d,继代周期20～30d,愈伤组织暗培养.在MS+1mg/L 2,4-D+0.5mg/L 6-BA培养基中,外植体脱分化率达100%.在MS+2.0mg/L 2,4-D+0.4mg/L 6-BA培养基中,1～4代愈伤组织增殖倍数达3.38;在MS+5mg/L 6-BA+0.1mg/L NAA中,60d不定芽诱导率达83.33%.在MS+3mg/L 6-BA+1mg/L NAA中,不定芽1～6代平均繁殖系数达6.78;不定芽用0.01%IBA处理40min后在1/2MS培养基中生根率达100%;85株试管苗移栽到田间土营养钵中,15d成活81株,成活率达95.29%.
The stems of “Hongyang” kiwifruit were used as explants. MS was used as basic medium. The culture temperature was 25 ± 2 ℃, light intensity was 4 500lx, 16h / d, and the subculture cycle was 20-30 days. The explant dedifferentiation rate was 100% in MS + 1mg / L 2,4-D + 0.5mg / L 6-BA medium.When MS + 2.0mg / L 2,4-D + 0.4mg / L In 6-BA medium, the multiplication rate of calluses from 1 to 4 generations reached 3.38, and the induction rate of adventitious shoots on 60d was 83.33% in MS + 5mg / L 6-BA + 0.1mg / L NAA. The average multiplication coefficient of adventitious buds from 1 to 6 generations was 6.78 in L 6-BA + 1 mg / L NAA. The rooting rate of adventitious buds in 1/2 MS medium was 100% after treated with 0.01% IBA for 40 min. Soil planted to the field of nutrition bowl, 15d survive 81, the survival rate of 95.29%.