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目的研究采用人巨细胞病毒(MCMV)诱导的实验性肝病新生小鼠模型,探讨高迁移率族蛋白1(HMGB1)在实验性新生小鼠肝炎肝脏组织中的表达及其意义。方法将48只BALB/c新生小鼠随机分为正常对照组(NC组)和病毒组(MCMV组),每组各24只,MCMV组应用新生小鼠一次性单侧腹腔注射MCMV 20μl方法建立肝病新生小鼠模型,NC组腹腔注射等量无菌生理盐水,分别于注射后3 d、7 d、14 d留取静脉血与肝脏组织(各8只),用HE染色观察肝脏病理变化;RT-PCR法和免疫组化法检测肝组织的HMGB1表达;ELISA法检测血清谷丙转氨酶(ALT)含量。结果 NC组HMGB1、ALT表达量在感染后第3天已明显升高,第7天达高峰,第14天有所下降,与NC组比较,差异有统计学意义(P<0.05),HMGB1与ALT在3 d、7 d呈正相关(P<0.05)。结论此次研究以BALB/c新生小鼠为研究对象,成功建立了肝病新生小鼠模型,发现HMGB1可能在鼠巨细胞病毒(MCMV)感染新生小鼠肝炎的发生、发展中起到重要作用,具体机制待进一步研究。
Objective To study the neonatal mouse model of experimental liver disease induced by human cytomegalovirus (MCMV) and to investigate the expression and significance of high mobility group box 1 (HMGB1) in liver tissue of experimental neonatal mice. Methods 48 BALB / c neonatal mice were randomly divided into normal control group (NC group) and virus group (MCMV group), 24 in each group. MCMV group was established by single intraperitoneal injection of MCMV 20μl Newborn mice with liver disease were injected intraperitoneally with equal volume of sterile saline. The venous blood and liver tissues (8 rats in each group) were collected on the 3rd, 7th and 14th day after injection. The pathological changes of the liver were observed by HE staining. The expression of HMGB1 in liver tissue was detected by RT-PCR and immunohistochemistry. The content of alanine aminotransferase (ALT) in serum was detected by ELISA. Results The expression of HMGB1 and ALT in NC group was significantly increased on the 3rd day after infection and peaked on the 7th day, decreased on the 14th day. The difference between the NC group and the NC group was statistically significant (P <0.05) ALT at 3 d, 7 d was positively correlated (P <0.05). Conclusion In this study, BALB / c neonatal mice were successfully established neonatal mouse model of liver disease and found that HMGB1 may play an important role in the occurrence and development of murine cytomegalovirus (MCMV) infection in neonatal mice, Specific mechanism to be further studied.