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目的观察Ghrelin对离体大鼠胰岛分泌胰岛素的影响。方法将分离纯化的大鼠胰岛置于含不同浓度葡萄糖和Ghrelin的孵育液中孵育1h,应用放射免疫分析法测定孵育液中胰岛素浓度。结果葡萄糖浓度>5.6 mmol/L时,浓度≥10 nmol/L的Ghrelin对胰岛素分泌有明显抑制作用(P<0.05)。结论高浓度Ghrelin可显著抑制高浓度葡萄糖诱导的胰岛素分泌。
Objective To observe the effect of Ghrelin on insulin secretion in isolated rat islets. Methods The isolated and purified rat islets were incubated in different concentrations of glucose and Ghrelin incubation solution incubated for 1 h, radioimmunoassay was used to determine the concentration of insulin in the incubation solution. Results Ghrelin concentration> 10 nmol / L significantly inhibited insulin secretion (P <0.05) when the glucose concentration was> 5.6 mmol / L. Conclusion High concentration of Ghrelin can significantly inhibit the high concentration of glucose-induced insulin secretion.