目的探讨含CpG基序的寡脱氧核苷酸对人肺癌细胞株A549放射敏感性的影响。方法分别用不同浓度CpG ODN处理A549细胞,经β射线照射后,检测细胞增殖和集落形成情况,ELISA法检测细胞TNF-α、IL-12和INF-γ的分泌水平,G riess检测细胞NO的含量。结果 CpG ODN抑制A549细胞的增殖,并呈量效关系。CpG ODN(10μg/m l)联合β射线照射,抑制A549细胞的增殖作用最强,且增加了TNF-α、IL-12、INF-γ和NO的分泌。结论 CpG ODN对A549细胞有明显的放射增敏作用,其机制可能与CpG ODN抑制A549细胞增殖、增强TNF-α、IL-12、INF-γ和NO的分泌有关。 Objective To investigate the effect of CpG motif oligodeoxynucleotides on radiosensitivity of human lung cancer cell line A549. Methods A549 cells were treated with different concentrations of CpG ODN. Proliferation and colony formation of A549 cells were detected by β-ray irradiation. The levels of TNF-α, IL-12 and INF-γ were detected by ELISA. content. Results CpG ODN inhibited A549 cell proliferation and showed a dose-response relationship. CpG ODN (10μg / ml) combined with β-irradiation inhibited the proliferation of A549 cells most strongly and increased the secretion of TNF-α, IL-12, INF-γ and NO. Conclusions CpG ODN has a significant radiosensitization effect on A549 cells, which may be related to the inhibition of CpG ODN proliferation and the secretion of TNF-α, IL-12, INF-γ and NO by A549 cells.