1 日本早期工作1901年日本的 Ishiwata 首次分离到了家蚕软腐病病原——苏云金杆菌(Bt),并进行了菌株筛选、培养基等生物学特性以及环境的影响等基础性研究,由于惧怕 Bt 扩散到蚕场,影响蚕丝业的发展,政府对 Bt 的应用持谨慎态度。50、60年代,Bt 制剂在美国、法国上市时,也曾流入日本,但仍担心对家蚕造成为害,政府通过检疫手段,禁止进口国外菌株及其产品。2 60年代日本对 Bt 的研究 1 Early Japanese work In 1901, Japan’s Ishiwata first isolated the Bt strain of Bombyx mori and carried out basic research on the biological characteristics of the strain, the culture medium, and the environmental impact. Due to the fear of Bt diffusion To the silkworm farm, affecting the development of silk industry, the government cautious about the application of Bt. In the 1950s and 1960s, Bt preparations were also introduced into Japan when they were listed in the United States and France. However, Bt preparations were still feared for causing damage to silkworms. The government banned the import of foreign strains and their products through quarantine measures. Japan ’s Study of Bt in the 1960s