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目的探讨抗促黄体激素释放激素受体(luteinizing hormone-releasing hormone receptor,LHRHR)单克隆抗体4F3B10对人子宫内膜癌Ishikawa细胞增殖及凋亡的影响。方法利用半定量RT-PCR分析Ishikawa细胞中LHRHR基因m RNA的转录水平,以β2-M基因为内参标准,通过光密度定量扫描比较确定LHRHR基因量。采用CCK-8法检测4F3B10对Ishikawa细胞增殖的影响;利用Annexin V-FITC/PI双染,在流式细胞仪上检测4F3B10对Ishikawa细胞凋亡的影响。结果 Ishikawa细胞中LHRHR的基因量约为内参的67.23%。4F3B10对Ishikawa细胞的增殖具有抑制作用,且呈剂量依赖性;经半数抑制浓度的4F3B10作用Ishikawa细胞48 h后,细胞凋亡率较未经4F3B10作用的细胞显著升高(P<0.05)。结论 4F3B10可有效抑制Ishikawa细胞增殖,并可诱导其凋亡。
Objective To investigate the effect of anti-luteinizing hormone-releasing hormone receptor (LHRHR) monoclonal antibody 4F3B10 on proliferation and apoptosis of human endometrial carcinoma cell line Ishikawa. Methods Semi-quantitative RT-PCR was used to analyze the transcriptional level of LHRHR m RNA in Ishikawa cells. The amount of LHRHR gene was determined by quantitative optical density scan using β2-M gene as internal standard. The effect of 4F3B10 on the proliferation of Ishikawa cells was detected by CCK-8 assay. The effect of 4F3B10 on the apoptosis of Ishikawa cells was detected by flow cytometry with Annexin V-FITC / PI double staining. Results The gene quantity of LHRHR in Ishikawa cells was about 67.23% of the reference. 4F3B10 inhibited the proliferation of Ishikawa cells in a dose-dependent manner. The apoptosis rate of Ishikawa cells treated with 4F3B10 at 4F3B10 for 48 h was significantly higher than that of 4F3B10-treated cells (P <0.05). Conclusion 4F3B10 can effectively inhibit the proliferation of Ishikawa cells and induce its apoptosis.