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目的 观察重组腺病毒介导胞嘧啶脱氨酶 (CD)基因和人野生型 p5 3基因共转染对直肠癌细胞的杀伤作用。方法 用携带CD/p5 3、CD、p5 3基因腺病毒感染直肠癌细胞SW 837,计数瘤细胞集落数 ,采用四唑蓝比色 (MTT法 )检测瘤细胞存活率。于 6 0只裸鼠移植瘤内注射重组腺病毒、磷酸盐缓冲液 (PBS) ,测定肿瘤生长抑制率。结果 用CD/p5 3、CD、p5 3重组腺病毒感染SW 837细胞 ,加 5 FC后细胞集落形成分别为 :6、38、6 9。裸鼠移植肿瘤生长抑制率分别为 78.6 %、5 6 .5 %、2 2 .3 %。CD/p5 3重组腺病毒感染组肿瘤细胞集落形成和存活率下降最显著 (P <0 .0 1) ,对肿瘤的抑制作用最明显。结论 CD自杀基因与野生型p5 3基因共转染对肿瘤细胞有更强的杀伤作用
Objective To observe the killing effect of recombinant adenovirus mediated cytosine deaminase (CD) gene and human wild type p5 3 gene on rectal cancer cells. Methods Rectal cancer cells SW 837 were infected with adenovirus carrying CD / p5, CD, and p53 genes. The number of tumor cells was counted. The survival rate of tumor cells was detected by MTT assay. Sixty nude mice transplanted tumors were injected with recombinant adenovirus, phosphate buffered saline (PBS), and the rate of tumor growth inhibition was measured. Results SW837 cells were infected with CD / p5 3, CD5 and p5 3 recombinant adenovirus. The cell colony formation after addition of 5 FC was 6,38,69, respectively. The tumor growth inhibition rate of nude mice transplantation were 78.6%, 56.5%, 22.3% respectively. CD / p5 3 recombinant adenovirus infection of tumor cell colony formation and survival rate decreased the most significant (P <0.01), the most obvious inhibition of the tumor. Conclusion Co-transfection of CD suicide gene and wild-type p5 3 gene has a stronger killing effect on tumor cells