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取非糖尿病创面及糖尿病足溃疡皮肤进行Fb培养后,分为对照组、糖尿病足溃疡(DFUs)组及APS(10、40、160、640μg/mL)组。分别通过MTT比色法及细胞计数法观察各组1～9d吸光度(A)值和细胞数量的变化。采用SA-β-Gal染色阳性率评价糖尿病足溃疡Fb的老化情况。结果:与对照组Fb相比,糖尿病足溃疡Fb数量明显减少、细胞增殖能力减退;APS在10～160ug/ml浓度范围内可增加体外培养的糖尿病足溃疡Fb数量、促进细胞增殖,且呈量效、时效依赖关系;过高浓度(≥640ug/ml)的APS对Fb增殖产生抑制作用。糖尿病足溃疡处Fb SA-β-Gal阳性率较对照组高,而APS可以降低糖尿病足溃疡处SA-β-Gal活性。结论:APS可以促进溃疡Fb增殖、延缓细胞衰老。
Fib cells from non-diabetic wounds and diabetic foot ulcers were divided into control group, diabetic foot ulcers (DFUs) and APS (10, 40, 160, 640 μg / mL) groups. The changes of absorbance (A) value and number of cells in each group were observed by MTT assay and cell counting method. The positive rate of SA-β-Gal staining was used to evaluate the aging of Fb in diabetic foot ulcer. Results: Compared with the control group, the number of Fb in diabetic foot ulcers decreased significantly and the cell proliferation decreased. APS increased the number of Fb in diabetic foot ulcers cultured in the range of 10 ~ 160 ug / ml, and promoted the proliferation of cells Effect, aging-dependent relationship; too high concentration (≥ 640ug / ml) of APS Fb proliferation inhibited. The positive rate of Fb SA-β-Gal in diabetic foot ulcer was higher than that in control group, while APS could reduce the SA-β-Gal activity in diabetic foot ulcer. Conclusion: APS can promote the proliferation of ulcer Fb and delay cellular senescence.