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目的建立大鼠海马胶质细胞培养牵张损伤模型。方法提取出生时间24h到48h的SD大鼠海马组织行星形胶质细胞培养,采用CICⅡ型细胞损伤装置、根据Ellis方法建立改良的大鼠海马胶质细胞体外牵张损伤模型,损伤程度分轻、中、重三级。对照组不予损伤。分别在2h和24h检测细胞乳酸脱氢酶释放量,并通过碘化丙啶荧光染色观察细胞损伤情况。结果细胞培养液中乳酸脱氢酶释放量随着损伤程度加重而增高。PrI红染细胞数随着牵张损伤的程度增高而增加。结论本实验建立的牵张损伤模型使用方便,可重复性好,适于进行神经细胞机械性体外损伤的研究。
Objective To establish a rat model of stretch injury induced by glial cells in hippocampus. Methods The hippocampus of SD rats born at 24 hours to 48 hours after birth was cultured in astrocytes. The injury model of hippocampus glial cells was established by Ellis method using CIC Ⅱ cell injury device. Medium and heavy three levels. The control group was not damaged. The release of lactate dehydrogenase (LDH) was measured at 2h and 24h, respectively. The cell damage was observed by propidium iodide staining. Results The amount of lactate dehydrogenase released from cell culture increased with the severity of injury. The number of PrI-stained red cells increased with the extent of stretch injury. Conclusion The stretch injury model established in this study is easy to use and has good reproducibility. It is suitable for the study of mechanical injury of nerve cells in vitro.