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目的 :探讨细菌L型临床标本的检出率 ,将特异性强、敏感性高的免疫学技术引入细菌L型检测工作。方法 :用金黄色葡萄球菌 (金葡菌 )及其L型的特异性抗体作为第一抗体 ,以生物素 抗生物素免疫酶染色法在各种临床标本中检测相应抗原。结果 :此种方法简便、快速 ,细菌培养后 6h免疫酶染色即呈阳性 ,并发现金葡菌L型抗体的免疫酶染色与某些革兰阴性菌之间有交叉反应。结论 :如能制备出特异性L型抗体作为第一抗体 ,结合特异、敏感的免疫酶技术 ,可以提高临床标本中细菌L型的检出率及缩短检验时间 ,值得在检验工作中推广。
OBJECTIVE: To investigate the detection rate of bacterial L-type clinical specimens and introduce the immunological technique with high specificity and sensitivity into bacterial L-type detection. Methods: Staphylococcus aureus (Staphylococcus aureus) and its L-specific antibody were used as the primary antibodies, and the corresponding antigens were detected in various clinical samples by biotin-streptavidin-immunoenzyme staining. Results: The method was simple and rapid. Immuno-enzyme staining was positive at 6h after bacterial culture. It was found that there was a cross-reaction between the immune enzyme staining of L-form A of Staphylococcus aureus and some Gram-negative bacteria. CONCLUSIONS: If a specific L-type antibody can be prepared as the primary antibody and combined with specific and sensitive immune enzyme technology, the detection rate of bacterial L-type in clinical samples can be increased and the examination time can be shortened, which is worth to be popularized in the test work.