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为了明确钴对NaCl胁迫的缓解作用,本研究就CoCl2对不同浓度NaCl胁迫下大麦种子的萌发、幼苗生长及内源激素与相关生理指标的影响进行了探讨。结果表明,随着NaCl胁迫程度的加重,大麦种子发芽势、发芽率显著降低,芽与根的生长受到显著抑制。幼苗脱落酸(ABA)与丙二醛(MDA)大量积累,抗氧化酶活性提高。加入0.1mmol/L CoCl2显著提高了NaCl胁迫下大麦种子的发芽势与发芽率,促进了芽与根的生长,抑制了幼苗ABA的积累,同时提高了抗氧化酶活性,降低了MDA的含量,且在较严重NaCl胁迫下作用更明显。CoCl2提高了NaCl胁迫下幼苗(IAA+GA3)/ABA的值,减缓了活性氧(ROS)的积累与膜脂过氧化进程,对种子萌发及幼苗生长起到了促进作用,在一定程度上提高了大麦幼苗对NaCl胁迫的适应能力。
In order to clarify the mitigating effect of cobalt on NaCl stress, the effects of CoCl2 on seed germination, seedling growth, endogenous hormones and related physiological indices of barley under different NaCl stress concentrations were studied. The results showed that with the increasing of NaCl stress, the seed germination potential and germination rate of barley decreased significantly, and the growth of bud and root was significantly inhibited. Abscisic acid (ABA) and malondialdehyde (MDA) accumulated in a large amount and the activities of antioxidant enzymes increased. The addition of 0.1mmol / L CoCl2 significantly increased the germination potential and germination rate of barley seed under NaCl stress, promoted the growth of buds and roots, inhibited the accumulation of ABA, increased the activity of antioxidant enzymes and decreased the content of MDA, And the effect is more obvious under more serious NaCl stress. CoCl2 increased the values of IAA + GA3 / ABA under NaCl stress, slowed the accumulation of reactive oxygen species (ROS) and the membrane lipid peroxidation, and promoted the seed germination and seedling growth to a certain extent Adaptability of barley seedlings to NaCl stress.