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目的:探讨干细胞因子(stem cell factor,SCF,又称KIT配体)和白血病抑制因子(leukaemiainhibitory factor,LIF)对冻融后人原始卵泡体外培养生长发育的影响。方法:收集18例卵巢良性肿瘤手术患者的正常卵巢皮质,采用直接覆盖玻璃化冷冻法保存卵巢组织,冻融后分离卵泡,随机分成5组:对照组(A组)、10 ng/ml SCF组(B组)、10 ng/ml LIF组(C组)、10 ng/ml SCF+10 ng/ml LIF组(D组)和100 ng/ml SCF+10 ng/ml LIF组(E组),行体外培养,比较SCF和/或LIF对人原始卵泡生长发育的影响。结果:①随培养天数的增加,对照组卵泡直径增长缓慢,明显小于各实验组(P<0.05);D、E组中卵泡直径增长迅速,较B、C组差异有统计学意义(P<0.05)。②随着培养天数的增加,各组卵泡均能分泌激素。4 d后对照组卵泡激素水平增长缓慢,明显低于各实验组(P<0.05),而D、E组中卵泡激素分泌增长迅速,较B、C组差异有统计学意义(P<0.05),D、E组中卵泡激素增长速度相差不大,但在第4日差异有统计学意义(P<0.05)。结论:在体外培养液中添加SCF或LIF以及两者联合添加有利于促进卵泡的生长发育,提高E2分泌功能,且SCF和LIF联合其作用更加明显。
Objective: To investigate the effects of stem cell factor (SCF) and leukemia inhibitor factor (LIF) on the growth and development of human primordial follicles after freezing and thawing. Methods: Normal ovarian cortex was collected from 18 patients with ovarian benign tumor. The ovarian tissue was preserved by direct cover vitrification. The follicles were isolated after freezing and thawing. They were randomly divided into 5 groups: control group (A group), 10 ng / ml SCF group (Group B), 10 ng / ml LIF (group C), 10 ng / ml SCF + 10 ng / ml LIF (group D) and 100 ng / ml SCF + 10 ng / ml LIF In vitro culture, the effects of SCF and / or LIF on the growth and development of human primordial follicles were compared. Results: (1) With the increase of culture days, the diameter of follicles in control group increased slowly, which was significantly smaller than that in experimental groups (P <0.05). The diameter of follicles in groups D and E increased rapidly, the difference was statistically significant (P < 0.05). ② With the increase in the number of days of culture, follicles in each group can secrete hormones. The level of follicle-stimulating hormone in control group increased slowly 4 days later, which was significantly lower than that in each experimental group (P <0.05), while the secretion of follicle-stimulating hormone in groups D and E increased rapidly compared with that in group B and C (P <0.05) There was no significant difference in the growth rate of follicle-stimulating hormone among groups D and E, but the difference was statistically significant on the 4th day (P <0.05). CONCLUSION: Adding SCF or LIF in vitro and the combination of the two can promote the growth and development of follicles and enhance the secretion of E2, and the combination of SCF and LIF is more obvious.