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目的探究ZNF703(锌指蛋白703,zinc finger protein 703)对人甲状腺乳头状癌K1细胞凋亡的影响。方法体外培养甲状腺乳头状癌K1细胞,以慢病毒为载体的ZNF703-sh RNA转染K1细胞,实验分为:空白组(空白PBS)、阴性对照组(转染无义序列ZNF703-sh RNA-NC)、干扰组(转染ZNF703-sh RNA序列),转染48小时后,分别利用R e a l time PCR、流式细胞仪检测K1细胞的ZNF703 m RNA表达水平及细胞凋亡。结果转染后各组中ZNF703 m RNA的相对表达量分别为:空白对照组(0.98±0.32)、阴性对照组(0.95±0.62)、干扰组(0.14±0.13),干扰组低于空白组和阴性组(P<0.05),显著低于空白对照组;转染后空白组、对照组、干扰组的细胞凋亡百分比分别为5.97±0.16、6.04±0.35、15.32±0.18,干扰组高于空白组和对照组(P<0.05)。结论 ZNF703基因对甲状腺乳头状癌K1细胞有显著的增殖抑制作用,并能诱导其发生细胞凋亡。
Objective To investigate the effect of ZNF703 (zinc finger protein 703) on the apoptosis of human thyroid papillary carcinoma cell line K1. Methods K1 cells of papillary thyroid carcinoma were cultured in vitro. Lentivirus-transfected ZNF703-sh RNA was transfected into K1 cells. The experiment was divided into blank group (blank PBS), negative control group (transfected with nonsense sequence ZNF703-sh RNA- NC) and interference group (transfected with ZNF703-sh RNA sequence). After transfected for 48 hours, the expression of ZNF703 mRNA and the apoptosis of K1 cells were detected by R eal time PCR and flow cytometry respectively. Results The relative expression of ZNF703 mRNA in each group was 0.98 ± 0.32, 0.95 ± 0.62 and 0.14 ± 0.13 respectively in the control group and the interference group was lower than that in the blank group Negative group (P <0.05), which was significantly lower than that of the blank control group. The percentages of apoptosis in blank group, control group and interference group after transfection were 5.97 ± 0.16,6.04 ± 0.35 and 15.32 ± 0.18 respectively, Group and control group (P <0.05). Conclusion ZNF703 gene can significantly inhibit the proliferation of papillary thyroid carcinoma K1 cells and induce apoptosis of K1 cells.