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为明确利用Brock转育成的小麦抗白粉病品系3B529(京411*7//农大015/Brock,F6)抗性的遗传基础,将高感白粉病小麦品系薛早和3B529杂交,获得F1代、F2分离群体和F2:3家系。抗病性鉴定和遗传分析结果表明,3B529对E09小种的抗性受1对显性基因控制,暂被定名为MlBrock。利用BSA和分子标记分析,获得了与MlBrock连锁的3个SSR标记Xcfd81、Xcfd78、Xgwm159和2个SCAR标记SCAR203和SCAR112,根据SSR和SCAR标记在中国春缺体-四体、双端体和缺失系的定位结果,将MlBrock定位在小麦染色体臂5DS Bin0～0.63区间上。MlBrock与Xcfd81和SCAR203共分离,与SCAR112的遗传距离为0.5cM。这些分子标记的建立有利于今后Brock抗白粉病基因分子标记辅助选择和基因聚合。综合抗白粉病基因MlBrock的染色体定位和抗谱分析结果,推测MlBrock很可能是Pm2基因。
In order to make clear the genetic basis of the resistance of wheat resistant line 3B529 (Beijing 411 * 7 // Nonghtong 015 / Brock, F6) transformed by Brock, F2 segregating population and F2: 3 pedigree. Disease resistance and genetic analysis showed that the resistance of 3B529 to race E09 was controlled by one pair of dominant genes and temporarily designated as MlBrock. Three SSR markers Xcfd81, Xcfd78, Xgwm159 and two SCAR markers SCAR203 and SCAR112 linked to MlBrock were obtained by BSA and molecular marker analysis. According to the SSR and SCAR markers, Department of the positioning results, the MlBrock located in wheat chromosome arm 5DS Bin0 ~ 0.63 range. MlBrock was co-segregated with Xcfd81 and SCAR203 and the genetic distance to SCAR112 was 0.5 cM. The establishment of these molecular markers is conducive to future Brock powdery mildew resistance gene molecular marker-assisted selection and gene polymerization. Based on the chromosomal location and anti-spectral analysis results of the powdery mildew resistance gene MlBrock, it is speculated that MlBrock is likely to be the Pm2 gene.