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整合因子复合体(integrator complex)是一个新近发现的多功能蛋白质复合物,由至少12个进化上保守的亚基(integrator complex subunits,INTS1~INTS12)组成。该复合体能与RNA聚合酶Ⅱ(RNAPⅡ)大亚基的具有重复氨基酸序列的C端相互作用,进而介导小核RNA(small nuclear RNA,snRNA)U1/U2的3’端加工。整合因子复合体还能通过与RNAPⅡ、延伸负调控因子NELF和转录延伸因子Spt5发生相互作用,调控NELF介导的RNAPⅡ的暂停-释放,进而调控mRNA的合成。近年来,有研究陆续报道整合因子复合体还在DNA损伤应答、核周动力蛋白的募集、细胞核内卡哈尔体(Cajal body)的完整性、脂肪细胞的分化成熟、造血、初级纤毛的形成、肿瘤的发生发展和病毒微小RNA(microRNA,miRNA)形成等方面发挥重要作用。该文综述了整合因子复合体的研究进展。
The integrator complex is a newly discovered multifunctional protein complex consisting of at least 12 evolutionary-conserved subunits (INTS1 to INTS12). This complex interacts with the C-terminal of the repetitive amino acid sequence of the RNA polymerase Ⅱ (Ⅱ) subunit, which in turn mediates the 3 ’end processing of the small nuclear RNA (snRNA) U1 / U2. The integration factor complex can also regulate the mRNA synthesis by regulating the NELF-mediated pause-release of RNAPII by interacting with RNAPⅡ, NELF, an elongation elongation factor and Spt5. In recent years, studies have reported that the integration factor complex is still in the DNA damage response, the recruitment of nuclear motility protein, the integrity of the nucleus Cajal body, the differentiation and maturation of adipocytes, hematopoiesis, the formation of primary cilia , The development of tumors and the formation of microRNAs (miRNAs) play an important role. This article reviews the research progress of integration factor complex.